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AB318257

Recombinant Mouse IL-1F10 protein (His tag)

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Recombinant Mouse IL-1F10 protein (His tag) is a Mouse Full Length protein, in the 2 to 152 aa range, expressed in Escherichia coli, with >95%, <0.005 EU/µg endotoxin level, suitable for Mass Spec, HPLC, SDS-PAGE.

View Alternative Names

Interleukin-1 family member 10, IL-1F10, Il1f10

3 Images
Mass Spectrometry - Recombinant Mouse IL-1F10 protein (His tag) (AB318257)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Mouse IL-1F10 protein (His tag) (AB318257)

Mass determination by ESI-TOF. Predicted MW is 18360.00 Da (+/- 10 Da by ESI-TOF). Observed MW is 18225.02 Da.

SDS-PAGE - Recombinant Mouse IL-1F10 protein (His tag) (AB318257)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse IL-1F10 protein (His tag) (AB318257)

SDS-PAGE analysis of ab318257 under reducing conditions for 2ug protein.

HPLC - Recombinant Mouse IL-1F10 protein (His tag) (AB318257)
  • HPLC

Supplier Data

HPLC - Recombinant Mouse IL-1F10 protein (His tag) (AB318257)

HPLC analysis of ab318257

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

HPLC, Mass Spec, SDS-PAGE

applications

Biologically active

No

Mass Spectrometry

LC-MS/MS

Accession

Q8R459

Animal free

Yes

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 Constituents: 10% PBS, 6.4993% Trehalose

storage-buffer

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Complementary Products

Primary Antibodies

AB180898

Anti-IL-1F10 antibody - C-terminal

Western blot - Anti-IL-1F10 antibody - C-terminal (AB180898)

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Recombinant human TNF alpha protein (Active)

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Flow Cytometry - Recombinant human TNF alpha protein (Active) (AB259410)

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Secondary Antibodies

AB150155

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

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Proteins & Peptides

AB50036

Recombinant human TGF beta 1 protein (Active)

Bioactive

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
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Sequence info

[{"sequence":"CSLPMARYYIIKDAHQKALYTRNGQLLLGDPDSDNYSPEKVCILPNRGLDRSKVPIFLGMQGGSCCLACVKTREGPLLQLEDVNIEDLYKGGEQTTRFTFFQRSLGSAFRLEAAACPGWFLCGPAEPQQPVQLTKESEPSTHTEFYFEMSR","proteinLength":"Full Length","predictedMolecularWeight":"18.36 kDa","actualMolecularWeight":"18.23 kDa","aminoAcidEnd":152,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q8R459","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
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Specifications

Form

Lyophilized

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General info

Function

Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2 (By similarity).

Sequence similarities

Belongs to the IL-1 family.

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Product protocols

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Target data

Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2 (By similarity).
See full target information Il1f10
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Product promise

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