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Recombinant Mouse IL-1F10 protein (His tag) is a Mouse Full Length protein, in the 2 to 152 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC.

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Images

Mass Spectrometry - Recombinant Mouse IL-1F10 protein (His tag) (AB318257), expandable thumbnail
  • HPLC - Recombinant Mouse IL-1F10 protein (His tag) (AB318257), expandable thumbnail

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

MS, HPLC

Biologically active

No

Mass spectrometry

LC-MS/MS

Amino acid sequence

C S L P M A R Y Y I I K D A H Q K A L Y T R N G Q L L L G D P D S D N Y S P E K V C I L P N R G L D R S K V P I F L G M Q G G S C C L A C V K T R E G P L L Q L E D V N I E D L Y K G G E Q T T R F T F F Q R S L G S A F R L E A A A C P G W F L C G P A E P Q Q P V Q L T K E S E P S T H T E F Y F E M S R

Reactivity data

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2 (By similarity).

Alternative names

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Recombinant Mouse IL-1F10 protein (His tag) is a Mouse Full Length protein, in the 2 to 152 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC.

Alternative names

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

Escherichia coli

Applications

MS, HPLC

Accession
Q8R459-1
Animal free

Yes

Species

Mouse

Concentration
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Storage buffer

pH: 7.4
Constituents: 10% PBS, 6.4993% Trehalose

Sequence info

Amino acid sequence

C S L P M A R Y Y I I K D A H Q K A L Y T R N G Q L L L G D P D S D N Y S P E K V C I L P N R G L D R S K V P I F L G M Q G G S C C L A C V K T R E G P L L Q L E D V N I E D L Y K G G E Q T T R F T F F Q R S L G S A F R L E A A A C P G W F L C G P A E P Q Q P V Q L T K E S E P S T H T E F Y F E M S R

Accession

Q8R459

Protein length

Full Length

Predicted molecular weight

18.36 kDa

Amino acids

2 to 152

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Lyophilized

General info

Function

Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2 (By similarity).

Sequence similarities

Belongs to the IL-1 family.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Notes

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Mass Spectrometry - Recombinant Mouse IL-1F10 protein (His tag) (ab318257), expandable thumbnail

    Mass Spectrometry - Recombinant Mouse IL-1F10 protein (His tag) (ab318257)

    Mass determination by ESI-TOF.
    Predicted MW is 18360.00 Da (+/- 10 Da by ESI-TOF). Observed MW is 18225.02 Da.

  • HPLC - Recombinant Mouse IL-1F10 protein (His tag) (ab318257), expandable thumbnail

    HPLC - Recombinant Mouse IL-1F10 protein (His tag) (ab318257)

    HPLC analysis of ab318257

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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