Recombinant Mouse IL-1F10 protein (His tag) is a Mouse Full Length protein, in the 2 to 152 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC, SDS-PAGE.
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- MS, HPLC, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
C S L P M A R Y Y I I K D A H Q K A L Y T R N G Q L L L G D P D S D N Y S P E K V C I L P N R G L D R S K V P I F L G M Q G G S C C L A C V K T R E G P L L Q L E D V N I E D L Y K G G E Q T T R F T F F Q R S L G S A F R L E A A A C P G W F L C G P A E P Q Q P V Q L T K E S E P S T H T E F Y F E M S R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2 (By similarity).
Alternative names
Interleukin-1 family member 10, IL-1F10, Il1f10
Recommended products
Recombinant Mouse IL-1F10 protein (His tag) is a Mouse Full Length protein, in the 2 to 152 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- MS, HPLC, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- Q8R459-1
- Animal free
- Yes
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10% PBS, 6.4993% Trehalose
Sequence info
Amino acid sequence
- C S L P M A R Y Y I I K D A H Q K A L Y T R N G Q L L L G D P D S D N Y S P E K V C I L P N R G L D R S K V P I F L G M Q G G S C C L A C V K T R E G P L L Q L E D V N I E D L Y K G G E Q T T R F T F F Q R S L G S A F R L E A A A C P G W F L C G P A E P Q Q P V Q L T K E S E P S T H T E F Y F E M S R
- Accession
- Q8R459
- Protein length
- Full Length
- Predicted molecular weight
- 18.36 kDa
- Actual molecular weight
- 18.23 kDa
- Amino acids
- 2 to 152
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2 (By similarity).
- Sequence similarities
Belongs to the IL-1 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Notes
Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant Mouse IL-1F10 protein (His tag) (ab318257)
SDS-PAGE analysis of ab318257 under reducing conditions for 2ug protein.
Mass Spectrometry - Recombinant Mouse IL-1F10 protein (His tag) (ab318257)
Mass determination by ESI-TOF.
Predicted MW is 18360.00 Da (+/- 10 Da by ESI-TOF). Observed MW is 18225.02 Da.
HPLC - Recombinant Mouse IL-1F10 protein (His tag) (ab318257)
HPLC analysis of ab318257
Downloads
Product protocols
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