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AB264457

Recombinant mouse IL-1ra/IL-1F3 protein (Active) (His tag N-Terminus)

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Recombinant mouse IL-1ra/IL-1F3 protein (Active) (His tag N-Terminus) is a Mouse Fragment protein, in the 27 to 178 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec.

View Alternative Names

Il-1ra, Il1rn, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor

2 Images
Functional Studies - Recombinant mouse IL-1ra/IL-1F3 protein (Active) (His tag N-Terminus) (AB264457)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse IL-1ra/IL-1F3 protein (Active) (His tag N-Terminus) (AB264457)

Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/ml with Mouse IL-1 alpha.

SDS-PAGE - Recombinant mouse IL-1ra/IL-1F3 protein (Active) (His tag N-Terminus) (AB264457)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse IL-1ra/IL-1F3 protein (Active) (His tag N-Terminus) (AB264457)

SDS-PAGE - Recombinant mouse IL-1ra/IL-1F3 protein (Active) (3 μg ab264457).

Visualized using Coomassie blue.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec, FuncS

applications

Biologically active

Yes

Biological activity

Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/ml with Mouse IL-1 alpha.

Accession

P25085

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMRPSGKRPCKMQAFRIWDTNQKTFYLRNNQLIAGYLQGPNIKLEEKIDMVPIDLHSVFLGIHGGKLCLSCAKSGDDIKLQLEEVNITDLSKNKEEDKRFTFIRSEKGPTTSFESAACPGWFLCTTLEADRPVSLTNTPEEPLIVTKFYFQEDQ","proteinLength":"Fragment","predictedMolecularWeight":"20.04 kDa","actualMolecularWeight":null,"aminoAcidEnd":178,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P25085","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True

Specifications

Form

Liquid

Additional notes

nan

General info

Function

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.

Sequence similarities

Belongs to the IL-1 family.

Product protocols

Target data

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
See full target information Il1rn

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