Recombinant mouse IL-1ra/IL-1F3 protein (Active) is a Mouse Fragment protein, in the 27 to 178 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS, MS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M R P S G K R P C K M Q A F R I W D T N Q K T F Y L R N N Q L I A G Y L Q G P N I K L E E K I D M V P I D L H S V F L G I H G G K L C L S C A K S G D D I K L Q L E E V N I T D L S K N K E E D K R F T F I R S E K G P T T S F E S A A C P G W F L C T T L E A D R P V S L T N T P E E P L I V T K F Y F Q E D Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
Alternative names
Il-1ra, Il1rn, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor
Recommended products
Recombinant mouse IL-1ra/IL-1F3 protein (Active) is a Mouse Fragment protein, in the 27 to 178 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS, MS
- Biologically active
- Yes
- Biological activity
- Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/ml with Mouse IL-1 alpha.
- Accession
- P25085-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M R P S G K R P C K M Q A F R I W D T N Q K T F Y L R N N Q L I A G Y L Q G P N I K L E E K I D M V P I D L H S V F L G I H G G K L C L S C A K S G D D I K L Q L E E V N I T D L S K N K E E D K R F T F I R S E K G P T T S F E S A A C P G W F L C T T L E A D R P V S L T N T P E E P L I V T K F Y F Q E D Q
- Accession
- P25085
- Protein length
- Fragment
- Predicted molecular weight
- 20.04 kDa
- Amino acids
- 27 to 178
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
nan
General info
- Function
Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
- Sequence similarities
Belongs to the IL-1 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Recombinant mouse IL-1ra/IL-1F3 protein (Active) (ab264457)
Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/ml with Mouse IL-1 alpha.
SDS-PAGE - Recombinant mouse IL-1ra/IL-1F3 protein (Active) (ab264457)
SDS-PAGE - Recombinant mouse IL-1ra/IL-1F3 protein (Active) (3 μg ab264457).
Visualized using Coomassie blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com