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AB283475

Recombinant Mouse IL-1RA protein (Active)

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(1 Publication)

Recombinant Mouse IL-1RA protein (Active) is a Mouse Full Length protein, in the 27 to 178 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, FuncS.

View Alternative Names

Il-1ra, Il1rn, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor

4 Images
Biological Activity - Recombinant Mouse IL-1RA protein (Active) (AB283475)
  • Biological Activity

Supplier Data

Biological Activity - Recombinant Mouse IL-1RA protein (Active) (AB283475)

Fully biologically active determined by its ability to inhibit IL-1 alpha dependent proliferation of D10S cells. ED50 ≤ 93.17 ng/ml, corresponding to a specific activity of 1.07 x 10^4 units/mg.

Mass Spectrometry - Recombinant Mouse IL-1RA protein (Active) (AB283475)
  • Mass Spec

Lab

Mass Spectrometry - Recombinant Mouse IL-1RA protein (Active) (AB283475)

Mass determination by ESI-TOF.

Predicted MW is 17388.87 Da (+/- 10 Da by ESI TOF). Observed MW is 17389.32 Da.

HPLC - Recombinant Mouse IL-1RA protein (Active) (AB283475)
  • HPLC

Lab

HPLC - Recombinant Mouse IL-1RA protein (Active) (AB283475)

HPLC analysis of ab283475.

SDS-PAGE - Recombinant Mouse IL-1RA protein (Active) (AB283475)
  • SDS-PAGE

Lab

SDS-PAGE - Recombinant Mouse IL-1RA protein (Active) (AB283475)

SDS-page analysis of ab283475.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, HPLC, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by its ability to inhibit IL-1 alpha dependent proliferation of D10S cells. ED50 ≤ 93.17 ng/ml, corresponding to a specific activity of 1.07 x 104 units/mg.

Accession

P25085

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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Sequence info

[{"sequence":"RPSGKRPCKMQAFRIWDTNQKTFYLRNNQLIAGYLQGPNIKLEEKIDMVPIDLHSVFLGIHGGKLCLSCAKSGDDIKLQLEEVNITDLSKNKEEDKRFTFIRSEKGPTTSFESAACPGWFLCTTLEADRPVSLTNTPEEPLIVTKFYFQEDQ","proteinLength":"Full Length","predictedMolecularWeight":"17.39 kDa","actualMolecularWeight":"17.39 kDa","aminoAcidEnd":178,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P25085","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

Purity by HPLC =95%

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General info

Function

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.

Sequence similarities

Belongs to the IL-1 family.

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Product protocols

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Target data

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
See full target information Il1rn
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:206 PubMed39160534

2024

CB2 expression in mouse brain: from mapping to regulation in microglia under inflammatory conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Wanda Grabon,Anne Ruiz,Nadia Gasmi,Cyril Degletagne,Béatrice Georges,Amor Belmeguenai,Jacques Bodennec,Sylvain Rheims,Guillaume Marcy,Laurent Bezin
View all publications
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