Recombinant Mouse IL-1RA protein (Active) is a Mouse Full Length protein, in the 27 to 178 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, HPLC
- Biologically active
- Yes
Amino acid sequence
R P S G K R P C K M Q A F R I W D T N Q K T F Y L R N N Q L I A G Y L Q G P N I K L E E K I D M V P I D L H S V F L G I H G G K L C L S C A K S G D D I K L Q L E E V N I T D L S K N K E E D K R F T F I R S E K G P T T S F E S A A C P G W F L C T T L E A D R P V S L T N T P E E P L I V T K F Y F Q E D Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
Alternative names
Il-1ra, Il1rn, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor
Recommended products
Recombinant Mouse IL-1RA protein (Active) is a Mouse Full Length protein, in the 27 to 178 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by its ability to inhibit IL-1 alpha dependent proliferation of D10S cells. ED50 ≤ 93.17 ng/ml, corresponding to a specific activity of 1.07 x 104 units/mg.
- Accession
- P25085-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- R P S G K R P C K M Q A F R I W D T N Q K T F Y L R N N Q L I A G Y L Q G P N I K L E E K I D M V P I D L H S V F L G I H G G K L C L S C A K S G D D I K L Q L E E V N I T D L S K N K E E D K R F T F I R S E K G P T T S F E S A A C P G W F L C T T L E A D R P V S L T N T P E E P L I V T K F Y F Q E D Q
- Accession
- P25085
- Protein length
- Full Length
- Predicted molecular weight
- 17.39 kDa
- Actual molecular weight
- 17.39 kDa
- Amino acids
- 27 to 178
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity by HPLC =95%
General info
- Function
Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
- Sequence similarities
Belongs to the IL-1 family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
SDS-PAGE - Recombinant Mouse IL-1RA protein (Active) (ab283475)
SDS-page analysis of ab283475.
Mass Spectrometry - Recombinant Mouse IL-1RA protein (Active) (ab283475)
Mass determination by ESI-TOF.
Predicted MW is 17388.87 Da (+/- 10 Da by ESI TOF). Observed MW is 17389.32 Da.
HPLC - Recombinant Mouse IL-1RA protein (Active) (ab283475)
HPLC analysis of ab283475.
Biological Activity - Recombinant Mouse IL-1RA protein (Active) (ab283475)
Fully biologically active determined by its ability to inhibit IL-1 alpha dependent proliferation of D10S cells. ED50 ≤ 93.17 ng/ml, corresponding to a specific activity of 1.07 x 10^4 units/mg.
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Product protocols
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