Recombinant Mouse IL-1RA protein (Active) is a Mouse Full Length protein, in the 27 to 178 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
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Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, MS, HPLC
- Biologically active
Yes
Amino acid sequence
R P S G K R P C K M Q A F R I W D T N Q K T F Y L R N N Q L I A G Y L Q G P N I K L E E K I D M V P I D L H S V F L G I H G G K L C L S C A K S G D D I K L Q L E E V N I T D L S K N K E E D K R F T F I R S E K G P T T S F E S A A C P G W F L C T T L E A D R P V S L T N T P E E P L I V T K F Y F Q E D Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
Alternative names
Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, Il1rn, Il-1ra
Recommended products
Recombinant Mouse IL-1RA protein (Active) is a Mouse Full Length protein, in the 27 to 178 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Alternative names
Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, Il1rn, Il-1ra
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, MS, HPLC
- Biologically active
Yes
- Biological activity
Fully biologically active determined by its ability to inhibit IL-1 alpha dependent proliferation of D10S cells. ED50 ≤ 93.17 ng/ml, corresponding to a specific activity of 1.07 x 104 units/mg.
- Accession
- P25085-1
- Animal free
Yes
- Species
Mouse
- Reconstitution
Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
R P S G K R P C K M Q A F R I W D T N Q K T F Y L R N N Q L I A G Y L Q G P N I K L E E K I D M V P I D L H S V F L G I H G G K L C L S C A K S G D D I K L Q L E E V N I T D L S K N K E E D K R F T F I R S E K G P T T S F E S A A C P G W F L C T T L E A D R P V S L T N T P E E P L I V T K F Y F Q E D Q
- Accession
- Protein length
Full Length
- Predicted molecular weight
17.39 kDa
- Actual molecular weight
17.39 kDa
- Amino acids
27 to 178
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
Purity by HPLC =95%
General info
- Function
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
- Sequence similarities
Belongs to the IL-1 family.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
SDS-PAGE - Recombinant Mouse IL-1RA protein (Active) (ab283475)
SDS-page analysis of ab283475.
Mass Spectrometry - Recombinant Mouse IL-1RA protein (Active) (ab283475)
Mass determination by ESI-TOF.
Predicted MW is 17388.87 Da (+/- 10 Da by ESI TOF). Observed MW is 17389.32 Da.
HPLC - Recombinant Mouse IL-1RA protein (Active) (ab283475)
HPLC analysis of ab283475.
Biological Activity - Recombinant Mouse IL-1RA protein (Active) (ab283475)
Fully biologically active determined by its ability to inhibit IL-1 alpha dependent proliferation of D10S cells. ED50 ≤ 93.17 ng/ml, corresponding to a specific activity of 1.07 x 10^4 units/mg.
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Product protocols
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