Recombinant mouse IL-2 protein (Active)

• FuncS

Lab

Functional Studies - Recombinant mouse IL-2 protein (Active) (AB259380)

Fully biologically active when compared to standard. Determined by the dose dependant proliferation of CTLL-2 cells. ED₅₀ is ≤ 0.5ng/mL, corresponding to a specific activity of 2.00 x 10⁶ units/mg.

• Mass Spec

Lab

Mass Spectrometry - Recombinant mouse IL-2 protein (Active) (AB259380)

M+/-0 Da, pass (+568/569 Da : Hex(1)HexNAc(2)) (Calc MW 17289.01)

The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water : FA (99.9 : 0.1 v/v) and 15 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) to 55 % water : FA (99.9 : 0.1 v/v) and 45 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).

• HPLC

Lab

HPLC - Recombinant mouse IL-2 protein (Active) (AB259380)

Purity ≥95%

The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water : TFA (99.9 : 0.1 v/v) and 20 % acetonitrile : water : TFA (90 : 9.9 : 0.1 v/v/v) to 20 % water : TFA (99.9 : 0.1 v/v) and 80 % acetonitrile : water : TFA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.

• SDS-PAGE

Lab

SDS-PAGE - Recombinant mouse IL-2 protein (Active) (AB259380)

SDS-PAGE analysis of ab259380.