Recombinant mouse IL-2 protein (Active)

4 product images

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  Functional Studies - Recombinant mouse IL-2 protein (Active) (ab259380)

  Fully biologically active when compared to standard. Determined by the dose dependant proliferation of CTLL-2 cells. ED₅₀ is ≤ 0.5ng/mL, corresponding to a specific activity of 2.00 x 10⁶ units/mg.

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  SDS-PAGE - Recombinant mouse IL-2 protein (Active) (ab259380)

  SDS-PAGE analysis of ab259380.

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  HPLC - Recombinant mouse IL-2 protein (Active) (ab259380)

  Purity ≥95%
  

  The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.

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  Mass Spectrometry - Recombinant mouse IL-2 protein (Active) (ab259380)

  M+/-0 Da, pass (+568/569 Da: Hex(1)HexNAc(2)) (Calc MW 17289.01)
  

  The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).