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AB280343

Recombinant Mouse IL-21 protein (Active)

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Recombinant Mouse IL-21 protein (Active) is a Mouse Full Length protein, in the 18 to 146 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, Cell Culture.

View Alternative Names

Interleukin-21, IL-21, Il21

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Functional Studies - Recombinant Mouse IL-21 protein (Active) (AB280343)
  • FuncS

Unknown

Functional Studies - Recombinant Mouse IL-21 protein (Active) (AB280343)

Fully biological active determined by its ability to enhance IFNɣ secretion in presence of human IL-15 in human KHYG-1 cells. ED50 for this effect is ≤7.9 ng/ml, corresponding to a specific activity of 1.25x105 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot GR3380830-2

Mass Spectrometry - Recombinant Mouse IL-21 protein (Active) (AB280343)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Mouse IL-21 protein (Active) (AB280343)

Mass determination by ESI-TOF.

Predicted MW is 15021.53 Da (+/- 10 Da by ESI-TOF). Observed mass is 15021.80 Da.

HPLC - Recombinant Mouse IL-21 protein (Active) (AB280343)
  • HPLC

Supplier Data

HPLC - Recombinant Mouse IL-21 protein (Active) (AB280343)

HPLC analysis of ab280343.

SDS-PAGE - Recombinant Mouse IL-21 protein (Active) (AB280343)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse IL-21 protein (Active) (AB280343)

SDS-PAGE analysis of ab280343.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

HPLC, Mass Spec, SDS-PAGE, Cell Culture

applications

Biologically active

Yes

Biological activity

Fully biological active determined by its ability to enhance IFNɣ secretion in presence of human IL-15 in human KHYG-1 cells. ED50 for this effect is ≤7.9 ng/ml, corresponding to a specific activity of 1.25x105 units/mg.

Accession

Q9ES17

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"HKSSPQGPDRLLIRLRHLIDIVEQLKIYENDLDPELLSAPQDVKGHCEHAAFACFQKAKLKPSNPGNNKTFIIDLVAQLRRRLPARRGGKKQKHIAKCPSCDSYEKRTPKEFLERLKWLLQKMIHQHLS","proteinLength":"Full Length","predictedMolecularWeight":"15.02 kDa","actualMolecularWeight":"15.02 kDa","aminoAcidEnd":146,"aminoAcidStart":18,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9ES17","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

=95%  by HPLC

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General info

Function

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (PubMed : 15100251). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15 (PubMed : 11970879). May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (PubMed : 12893770).

Sequence similarities

Belongs to the IL-15/IL-21 family.

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Product protocols

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Target data

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (PubMed : 15100251). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15 (PubMed : 11970879). May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (PubMed : 12893770).
See full target information Il21
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