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AB281809

Recombinant mouse IL-22 protein (Active)

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(1 Publication)

Recombinant mouse IL-22 protein (Active) is a Mouse Full Length protein, in the 25 to 179 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

View Alternative Names

Il22a, Iltif, Iltifa, Il22, Interleukin-22, IL-22, IL-10-related T-cell-derived-inducible factor, IL-TIF alpha, Interleukin-22a, IL-TIF, IL-22a

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Mass Spectrometry - Recombinant mouse IL-22 protein (Active) (AB281809)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant mouse IL-22 protein (Active) (AB281809)

Mass determination by ESI-TOF.

Predicted MW is 16697.32 Da. (+/- 10 Da by ESI-TOF). Observes MW is 16700.44 Da.

Functional Studies - Recombinant mouse IL-22 protein (Active) (AB281809)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse IL-22 protein (Active) (AB281809)

Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells.

ED50 is ≤530 pg/ml, corresponding to a specific activity of 1.88 x 106 units /mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3384879-2

SDS-PAGE - Recombinant mouse IL-22 protein (Active) (AB281809)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse IL-22 protein (Active) (AB281809)

SDS-PAGE analysis of ab281809.

HPLC - Recombinant mouse IL-22 protein (Active) (AB281809)
  • HPLC

Supplier Data

HPLC - Recombinant mouse IL-22 protein (Active) (AB281809)

HPLC analysis of ab281809.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, HPLC, Mass Spec, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells.

ED50 is ≤530 pg/ml, corresponding to a specific activity of 1.88 x 106 units /mg.

Accession

Q9JJY9

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"LPVNTRCKLEVSNFQQPYIVNRTFMLAKEASLADNNTDVRLIGEKLFRGVSAKDQCYLMKQVLNFTLEDVLLPQSDRFQPYMQEVVPFLTKLSNQLSSCHISGDDQNIQKNVRRLKETVKKLGESGEIKAIGELDLLFMSLRNACV","proteinLength":"Full Length","predictedMolecularWeight":"16.69 kDa","actualMolecularWeight":"16.7 kDa","aminoAcidEnd":179,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9JJY9","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

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General info

Function

Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed : 33852830, PubMed : 35525330). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed : 33912578). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB. Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways. Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (PubMed : 24742671). Promotes epithelial cell spreading (PubMed : 24742671).

Sequence similarities

Belongs to the IL-10 family.

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Product protocols

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Target data

Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed : 33852830, PubMed : 35525330). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed : 33912578). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB. Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways. Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (PubMed : 24742671). Promotes epithelial cell spreading (PubMed : 24742671).
See full target information Il22
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Viruses 17: PubMed40006939

2025

ILC3 Function as a Double-Edged Sword in EV71 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Zhang,Linlin Bao,Feifei Qi,Qi Lv,Fengdi Li,Chuan Qin
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