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AB119155

Recombinant mouse IL-31 protein

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Recombinant mouse IL-31 protein is a Mouse Full Length protein, in the 24 to 163 aa range, expressed in Escherichia coli, with >95%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, FuncS.

View Alternative Names

Interleukin-31, IL-31, Il31

2 Images
Western blot - Recombinant mouse IL-31 protein (AB119155)
  • WB

Unknown

Western blot - Recombinant mouse IL-31 protein (AB119155)

ab119155 used in Western Blot. Figure : 1 ug run under (+) reducing conditions and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Mouse IL-31 has a predicted MW of 15.7 kDa.

All lanes:

Western blot - Recombinant mouse IL-31 protein (ab119155)

false

SDS-PAGE - Recombinant mouse IL-31 protein (AB119155)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant mouse IL-31 protein (AB119155)

SDS PAGE analysis of ab119155 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

The activity is determined by the dose dependent production of IL-8 from human PBMCs and is typically less than 25 ng/mL.

Accession

Q6EAL8

Animal free

No

Carrier free

Yes

Species

Mouse

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.14% Sodium phosphate

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.

Sequence info

[{"sequence":"MTCSLSFGAPISKEDLRTTIDLLKQESQDLYNNYSIKQASGMSADESIQLPCFSLDREALTNISVIIAHLEKVKVLSENTVDTSWVIRWLTNISCFNPLNLNISVPGNTDESYDCKVFVLTVLKQFSNCMAELQAKDNTTC","proteinLength":"Full Length","predictedMolecularWeight":"15.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":163,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6EAL8","tags":[]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Reconstitute for long term storage
True

Specifications

Form

Lyophilized

Additional notes

Protein content and purity of ab119155 was determined by UV spectroscopy at 280 nm, RP-HPLC calibrated against a known standard and quantitation against a known standard via reducing and non-reducing SDS-PAGE gels. Purified using standard chromatographical techniques.

General info

Function

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR (PubMed : 15184896). May function in skin immunity (PubMed : 15184896). Enhances myeloid progenitor cell survival in vitro (PubMed : 17379091). Induces RETNLA and serum amyloid A protein expression in macrophages (PubMed : 25847241).

Product protocols

Target data

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR (PubMed : 15184896). May function in skin immunity (PubMed : 15184896). Enhances myeloid progenitor cell survival in vitro (PubMed : 17379091). Induces RETNLA and serum amyloid A protein expression in macrophages (PubMed : 25847241).
See full target information Il31

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