Recombinant mouse IL-33 protein (Active) is a Mouse Full Length protein in the 109 to 266 aa range with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies, mass spectrometry, Cell Culture and HPLC.The predicted molecular weight of ab270066 protein is 17.62 kDa.
- Save time and ensure accurate results - use our recombinant mouse IL-33 as a control
- Optimal protein bioactivity and stability
- Available in different sizes to fit your experimental needs
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, Cell Culture, HPLC
- Biologically active
- Yes
Amino acid sequence
S I Q G T S L L T Q S P A S L S T Y N D Q S V S F V L E N G C Y V I N V D D S G K D Q E Q D Q V L L R Y Y E S P C P A S Q S G D G V D G K K L M V N M S P I K D T D I W L H A N D K D Y S V E L Q R G D V S P P E Q A F F V L H K K S S D F V S F E C K N L P G T Y I G V K D N Q L A L V E E K D E S C N N I M F K L S K I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:29045903). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (By similarity). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (By similarity). Acts as an enhancer of polarization of alternatively activated macrophages (By similarity). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (PubMed:34644537). In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (By similarity). This form is rapidely lost upon angiogenic or pro-inflammatory activation (By similarity).
Alternative names
Interleukin-33, IL-33, Il33
Recommended products
Recombinant mouse IL-33 protein (Active) is a Mouse Full Length protein in the 109 to 266 aa range with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies, mass spectrometry, Cell Culture and HPLC.The predicted molecular weight of ab270066 protein is 17.62 kDa.
- Save time and ensure accurate results - use our recombinant mouse IL-33 as a control
- Optimal protein bioactivity and stability
- Available in different sizes to fit your experimental needs
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, Cell Culture, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active when compared to standard. Determined by the dose dependant proliferation of D10.G4.1 cells.
ED50 is ≤ 0.8747, corresponding to a specific activity of 1.14 x 106 units/mg.
- Accession
- Q8BVZ5-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute with Phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- S I Q G T S L L T Q S P A S L S T Y N D Q S V S F V L E N G C Y V I N V D D S G K D Q E Q D Q V L L R Y Y E S P C P A S Q S G D G V D G K K L M V N M S P I K D T D I W L H A N D K D Y S V E L Q R G D V S P P E Q A F F V L H K K S S D F V S F E C K N L P G T Y I G V K D N Q L A L V E E K D E S C N N I M F K L S K I
- Accession
- Q8BVZ5
- Protein length
- Full Length
- Predicted molecular weight
- 17.62 kDa
- Amino acids
- 109 to 266
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity =95% by HPLC.
General info
- Function
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:29045903). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (By similarity). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (By similarity). Acts as an enhancer of polarization of alternatively activated macrophages (By similarity). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (PubMed:34644537).
- Sequence similarities
Belongs to the IL-1 family. Highly divergent.
- Post-translational modifications
The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor (PubMed:16286016). However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full-length protein (PubMed:22307629, PubMed:35749514). May also be proteolytically processed by calpains. Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:16286016, PubMed:19465481). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase (PubMed:16286016, PubMed:19465481).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- -20°C, Ambient
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our recombinant mouse IL-33 protein ab270066 as a control.
The ab270066 mouse IL-33 protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC.
Check out our protein gel staining guide for SDS-PAGE here
Premium Bioactive Protein range
The ab270066 mouse IL-33 protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.
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4 product images
Functional Studies - Recombinant mouse IL-33 protein (Active) (ab270066)
Fully biologically active when compared to standard. Determined by the dose dependant proliferation of D10.G4.1 cells.
ED50 is ≤ 0.8747, corresponding to a specific activity of 1.14 x 106 units/mg.
SDS-PAGE - Recombinant mouse IL-33 protein (Active) (ab270066)
SDS-PAGE - Recombinant mouse IL-33 protein (Active) (ab270066).Purity ≥95%.
Mass Spectrometry - Recombinant mouse IL-33 protein (Active) (ab270066)
M+1.3 Da. Calc MW is17624.69 Da.
HPLC - Recombinant mouse IL-33 protein (Active) (ab270066)
Purity ≥95%.
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