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Recombinant mouse IL-4 protein (Active) is a Mouse Full Length protein, in the 21 to 140 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.

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Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, MS, HPLC, Cell Culture

Biologically active

Yes

Actual molecular weight

13.62 kDa

Sequence

H I H G C D K N H L R E I I G I L N E V T G E G T P C T E M D V P N V L T A T K N T T E S E L V C R A S K V L R I F Y L

K H G K T P C L K K N S S V L M E L Q R L F R A F R C L D S S I S C T M N E S K S T S L K D F L E S L K S I M Q M D Y S

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Cell Culture
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant mouse IL-4 protein (Active) is a Mouse Full Length protein, in the 21 to 140 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, MS, HPLC, Cell Culture

Biological activity

Fully biologically active determined by dose dependent increase in HT-2 cell proliferation.

ED50 is ≤1.04 ng/ml, corresponding to a specific activity of 0.96 x 106 units/mg.

Predicted molecular weight

13.62 kDa

Amino acids

21 to 140

Sequence

H I H G C D K N H L R E I I G I L N E V T G E G T P C T E M D V P N V L T A T K N T T E S E L V C R A S K V L R I F Y L

K H G K T P C L K K N S S V L M E L Q R L F R A F R C L D S S I S C T M N E S K S T S L K D F L E S L K S I M Q M D Y S

Accession
P07750-1
Protein length

Full Length

Animal free

Yes

Nature

Recombinant

Species

Mouse

Reconstitution

Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

Concentration
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Storage buffer

pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Specifications

Form

Lyophilized

Additional notes

=95%  by HPLC

General info

Function

Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3083412). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells (PubMed:3498301). It enhances both secretion and cell surface expression of IgE and IgG1 (PubMed:3498301). It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (PubMed:25847241). Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4 (PubMed:26416964).

Sequence similarities

Belongs to the IL-4/IL-13 family.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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