Recombinant mouse IL-5 protein (Active)
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Recombinant mouse IL-5 protein (Active) is a Mouse Full Length protein, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.
View Alternative Names
Il-5, Il5, Interleukin-5, IL-5, B-cell growth factor II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, BCGF-II, TRF
- FuncS
Supplier Data
Functional Studies - Recombinant mouse IL-5 protein (Active) (AB283426)
Fully biologically active determined by dose dependent proliferation of TF-1 cells.
ED50 for this effect is ≤ 0.25 ng/ml corresponding to specific activity of 4 X 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3395754-2
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant mouse IL-5 protein (Active) (AB283426)
Mass determination by ESI-TOF.
Predicted MW is 13183.29 Da. (+/- 10 Da by ESI-TOF). Observes MW is 13183.29 Da. Additional mass at 13185.29 is due to residual O-glycans.
- HPLC
Supplier Data
HPLC - Recombinant mouse IL-5 protein (Active) (AB283426)
HPLC analysis of ab283426
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant mouse IL-5 protein (Active) (AB283426)
SDS-PAGE analysis of ab283426
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Specifications
Form
Lyophilized
Additional notes
>=95% Purity by HPLC
General info
Function
Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 10444455, PubMed : 1873482). Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (PubMed : 3128631). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (By similarity).
Sequence similarities
Belongs to the IL-5 family.
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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