Recombinant Mouse IL-9 Protein (Fc Chimera) is a Mouse Full Length protein, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- MS, HPLC, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
Q R C S T T W G I R D T N Y L I E N L K D D P P S K C S C S G N V T S C L C L S V P T D D C T T P C Y R E G L L Q L T N A T Q K S R L L P V F H R V K R I V E V L K N I T C P S F S C E K P C N Q T M A G N T L S F L K S L L G T F Q K T E M Q R Q K S R P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites (PubMed:11070175, PubMed:19433802). Affects intestinal epithelial permeability and adaptive immunity (PubMed:12704113). In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells (PubMed:11070175, PubMed:19433802). Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG (PubMed:2145361, PubMed:7718508). Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs (PubMed:10464327). Induction of differentiation genes seems to be mediated by STAT1 alone, while protection of cells from apoptosis depends on STAT3 and STAT5 (PubMed:10464327).
Alternative names
Interleukin-9, IL-9, Cytokine P40, T-cell growth factor P40, Il9
Recommended products
Recombinant Mouse IL-9 Protein (Fc Chimera) is a Mouse Full Length protein, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, HPLC, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- MS, HPLC, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P15247-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Q R C S T T W G I R D T N Y L I E N L K D D P P S K C S C S G N V T S C L C L S V P T D D C T T P C Y R E G L L Q L T N A T Q K S R L L P V F H R V K R I V E V L K N I T C P S F S C E K P C N Q T M A G N T L S F L K S L L G T F Q K T E M Q R Q K S R P
- Accession
- P15247
- Protein length
- Full Length
- Predicted molecular weight
- 40.11 kDa
- Actual molecular weight
- 39.97 kDa
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites (PubMed:11070175, PubMed:19433802). Affects intestinal epithelial permeability and adaptive immunity (PubMed:12704113). In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells (PubMed:11070175, PubMed:19433802). Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG (PubMed:2145361, PubMed:7718508). Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs (PubMed:10464327). Induction of differentiation genes seems to be mediated by STAT1 alone, while protection of cells from apoptosis depends on STAT3 and STAT5 (PubMed:10464327).
- Sequence similarities
Belongs to the IL-7/IL-9 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interleukin-9 (IL-9) sometimes known as a T-cell growth factor is a cytokine with a molecular mass of approximately 14-19 kDa. It is primarily expressed by T helper cells particularly Th2 and Th9 cells. IL-9 comes from the IL-9 gene and plays a significant role in the immune system by affecting various cell types such as mast cells B cells and epithelial cells. Researchers first identified it in the late 1980s and it has continued to be an area of interest due to its diverse functions.
- Biological function summary
IL-9 influences immune responses and is important for various processes including cell proliferation and survival. IL-9 activates mast cells which play role in allergy response. It also affects the growth and differentiation of T cells and enhances the function of dendritic cells. IL-9 operates individually and not as part of a larger complex but works closely with its receptor IL-9R to initiate downstream signaling events.
- Pathways
Cytokine signaling pathways and JAK-STAT signaling pathways integrate IL-9's function in the immune system. IL-9 activates the JAK-STAT pathway particularly involving proteins like JAK1 and STATs to relay signals that promote immune cell growth and survival. This cytokine also interlinks with other cytokines such as IL-4 and IL-13 playing role in immune regulation and inflammatory responses.
- Associated diseases and disorders
IL-9 shows strong correlation with asthma and certain types of cancers particularly Hodgkin's lymphoma. In asthma overproduction of IL-9 can lead to increased mucus production and airway hyperresponsiveness. This relationship makes it a potential target for blocking therapies in asthma treatment. Higher levels of IL-9 correlate with poorer prognosis in some cancers indicating its potential role in tumor growth and immune evasion possibly interacting with IL-6 in such conditions.
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1 product image
SDS-PAGE - Recombinant Mouse IL-9 Protein (Fc Chimera) (ab322316)
SDS-PAGE analysis of ab322316 under reducing conditions for 2ug protein.
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