Recombinant Mouse IL36 gamma/IL-1F9 protein (Animal Free) is a Mouse Full Length protein, in the 13 to 164 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G R E T P D F G E V F D L D Q Q V W I F R N Q A L V T V P R S H R V T P V S V T I L P C K Y P E S L E Q D K G I A I Y L G I Q N P D K C L F C K E V N G H P T L L L K E E K I L D L Y H H P E P M K P F L F Y H T R T G G T S T F E S V A F P G H Y I A S S K T G N P I F L T S K K G E Y Y N I N F N L D I K S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (By similarity). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in pro-inflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of pro-inflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes.
Alternative names
Il1f9, Il36g, Interleukin-36 gamma, Interleukin-1 family member 9, IL-1F9
Recommended products
Recombinant Mouse IL36 gamma/IL-1F9 protein (Animal Free) is a Mouse Full Length protein, in the 13 to 164 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8R460-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute in 10 mM HCl
- Concentration
- Storage buffer
Constituents: 0.1% Trifluoroacetic acid
Sequence info
Amino acid sequence
- M G R E T P D F G E V F D L D Q Q V W I F R N Q A L V T V P R S H R V T P V S V T I L P C K Y P E S L E Q D K G I A I Y L G I Q N P D K C L F C K E V N G H P T L L L K E E K I L D L Y H H P E P M K P F L F Y H T R T G G T S T F E S V A F P G H Y I A S S K T G N P I F L T S K K G E Y Y N I N F N L D I K S
- Accession
- Q8R460
- Protein length
- Full Length
- Predicted molecular weight
- 17.5 kDa
- Amino acids
- 13 to 164
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity determined by reducing and non-reducing SDS-PAGE.
General info
- Function
Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (By similarity). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in pro-inflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of pro-inflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes.
- Sequence similarities
Belongs to the IL-1 family.
- Post-translational modifications
N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold) (PubMed:21965679). Proteolytically cleaved by cathepsin CTSG (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Previously labelled as IL36 gamma.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IL36 gamma also known as IL-1F9 or IL-36 is a member of the interleukin-1 cytokine family. The protein weighs approximately 22 kDa. It is expressed highly in epithelial tissues such as keratinocytes in the skin bronchial tissues and intestinal lining. It acts mainly on nearby cells to influence immune responses through cytokine signaling.
- Biological function summary
IL36 gamma plays a role in the immune and inflammatory responses. It operates as a cytokine that signals through the IL-1 receptor family leading to the activation of NF-kB and MAPK pathways. This member of the IL-1 family does not form a complex by itself but interacts with IL-36R to exert its functions. The protein influences processes such as cell proliferation differentiation and the regulation of immune responses to external pathogens or injuries.
- Pathways
IL36 gamma is involved in inflammatory and immune signaling pathways. Within the immune response pathway it stimulates the production of other cytokines and pro-inflammatory molecules. IL36 gamma is associated with the IL-1 signaling pathway alongside other cytokines such as IL-1 and IL-18 which all contribute to immune modulation and inflammatory responses.
- Associated diseases and disorders
IL36 gamma has connections to psoriasis and inflammatory bowel disease. In psoriasis increased levels of IL36 gamma correlate with heightened skin inflammation and proliferation of keratinocytes. In inflammatory bowel disease IL36 gamma contributes to the inflammatory state of the gut. The activities of IL36 gamma often link with other cytokines such as TNF-alpha which collectively drive the inflammatory processes observed in these conditions.
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SDS-PAGE - Recombinant Mouse IL36 gamma/IL-1F9 protein (Animal Free) (ab219124)
4-20% Tris-Glycine SDS-PAGE analysis of 1 μg ab219124, stained with Coomasie blue.
Lane 1: Non-reducing conditions.
Lane 2: Reducing conditions.
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