Recombinant Mouse IL36 gamma/IL-1F9 protein (His Tag) is a Mouse Full Length protein, in the 13 to 164 aa range, expressed in Escherichia coli, with <95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS.
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Key facts
- Purity
<95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
MS
- Biologically active
No
- Mass spectrometry
LC-MS/MS
Amino acid sequence
G R E T P D F G E V F D L D Q Q V W I F R N Q A L V T V P R S H R V T P V S V T I L P C K Y P E S L E Q D K G I A I Y L G I Q N P D K C L F C K E V N G H P T L L L K E E K I L D L Y H H P E P M K P F L F Y H T R T G G T S T F E S V A F P G H Y I A S S K T G N P I F L T S K K G E Y Y N I N F N L D I K S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (By similarity). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in proinflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes.
Alternative names
Interleukin-36 gamma, Interleukin-1 family member 9, IL-1F9, Il1f9, Il36g
Recommended products
Recombinant Mouse IL36 gamma/IL-1F9 protein (His Tag) is a Mouse Full Length protein, in the 13 to 164 aa range, expressed in Escherichia coli, with <95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS.
Alternative names
Interleukin-36 gamma, Interleukin-1 family member 9, IL-1F9, Il1f9, Il36g
Key facts
- Purity
<95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
MS
- Biologically active
No
- Mass spectrometry
LC-MS/MS
- Accession
- Q8R460-1
- Animal free
Yes
- Species
Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10% PBS, 6.4993% Trehalose
Sequence info
Amino acid sequence
G R E T P D F G E V F D L D Q Q V W I F R N Q A L V T V P R S H R V T P V S V T I L P C K Y P E S L E Q D K G I A I Y L G I Q N P D K C L F C K E V N G H P T L L L K E E K I L D L Y H H P E P M K P F L F Y H T R T G G T S T F E S V A F P G H Y I A S S K T G N P I F L T S K K G E Y Y N I N F N L D I K S
- Accession
- Protein length
Full Length
- Predicted molecular weight
20.36 kDa
- Actual molecular weight
20.23 kDa
- Amino acids
13 to 164
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (By similarity). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in proinflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes.
- Sequence similarities
Belongs to the IL-1 family.
- Post-translational modifications
N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold).
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
Ambient
- Appropriate long-term storage conditions
Ambient
Notes
Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
Supplementary info
- Activity summary
IL36 gamma also known as IL-1F9 or IL-36 is a member of the interleukin-1 cytokine family. The protein weighs approximately 22 kDa. It is expressed highly in epithelial tissues such as keratinocytes in the skin bronchial tissues and intestinal lining. It acts mainly on nearby cells to influence immune responses through cytokine signaling.
- Biological function summary
IL36 gamma plays a role in the immune and inflammatory responses. It operates as a cytokine that signals through the IL-1 receptor family leading to the activation of NF-kB and MAPK pathways. This member of the IL-1 family does not form a complex by itself but interacts with IL-36R to exert its functions. The protein influences processes such as cell proliferation differentiation and the regulation of immune responses to external pathogens or injuries.
- Pathways
IL36 gamma is involved in inflammatory and immune signaling pathways. Within the immune response pathway it stimulates the production of other cytokines and pro-inflammatory molecules. IL36 gamma is associated with the IL-1 signaling pathway alongside other cytokines such as IL-1 and IL-18 which all contribute to immune modulation and inflammatory responses.
- Associated diseases and disorders
IL36 gamma has connections to psoriasis and inflammatory bowel disease. In psoriasis increased levels of IL36 gamma correlate with heightened skin inflammation and proliferation of keratinocytes. In inflammatory bowel disease IL36 gamma contributes to the inflammatory state of the gut. The activities of IL36 gamma often link with other cytokines such as TNF-alpha which collectively drive the inflammatory processes observed in these conditions.
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Mass Spectrometry - Recombinant Mouse IL36 gamma/IL-1F9 protein (His Tag) (ab318254)
Mass determination by ESI-TOF.
Predicted MW is 20360.00 Da (+/- 10 Da by ESI-TOF). Observed MW is 20225.72Da.
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