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AB259386

Recombinant mouse Interferon alpha 1 protein (Active)

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Recombinant mouse Interferon alpha 1 protein (Active) is a Mouse Full Length protein, in the 24 to 189 aa range, expressed in HEK 293 cells, with >95%, < 0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

View Alternative Names

Interferon alpha-1, IFN-alpha-1, Ifna1

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Mass Spectrometry - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)

M-Arg + 0.3 Da (loss of C-terminal lysine during analysis). Calculated MW is 19175.70 Da.

Functional Studies - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)

Cytopathic Effect Inhibition Assay of L929 cells against Encephalomyocarditis virus.

ED50 < 7 x 10-3 ng/mL, corresponding to a specific activity of 1.43 x 108 units/mg.

SDS-PAGE - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)

SDS-PAGE analysis of ab259386.

HPLC - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)
  • HPLC

Supplier Data

HPLC - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)

Purity by HPLC ≥95%

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

HPLC, FuncS, SDS-PAGE, Mass Spec, Cell Culture

applications

Biologically active

Yes

Biological activity

Cytopathic Effect Inhibition Assay of L929 cells against Encephalomyocarditis virus.

ED50 < 7 x 10-3 ng/mL, corresponding to a specific activity of 1.43 x 108 units/mg.

Accession

P01572

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 6 - 8 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment.
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Sequence info

[{"sequence":"CDLPQTHNLRNKRALTLLVQMRRLSPLSCLKDRKDFGFPQEKVDAQQIKKAQAIPVLSELTQQILNIFTSKDSSAAWNTTLLDSFCNDLHQQLNDLQGCLMQQVGVQEFPLTQEDALLAVRKYFHRITVYLREKKHSPCAWEVVRAEVWRALSSSANVLGRLREEK","proteinLength":"Full Length","predictedMolecularWeight":"19.18 kDa","actualMolecularWeight":null,"aminoAcidEnd":189,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P01572","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon alpha 1 (IFN-α1) is a type I interferon with significant antiviral and immunomodulatory activities. Alternate names include interferon alpha and IFNA1. This protein weighs approximately 19 kDa and expresses predominantly in leukocytes. It interacts with specific cell surface receptors to initiate various protective mechanisms in the cell. Researchers often study IFN-alpha-1 protein using techniques like interferon alpha ELISA and IFN-α ELISA for its effective quantification in experimental analyses.
Biological function summary

Interferon alpha 1 functions as an important player in the immune response against viral infections. It belongs to a family of cytokines that signal through the JAK-STAT pathway to induce the expression of interferon-stimulated genes (ISGs). IFN-α1 operates independently and does not form large complexes with other proteins ensuring a focused response to pathogens. This protein's role is critical for the initiation of immune responses and limiting the spread of viruses within the host organism.

Pathways

Interferon alpha 1 activates intracellular signaling cascades mainly the JAK-STAT pathway and NF-κB pathway. These pathways have important roles in cellular defense mechanisms. In the JAK-STAT pathway IFN-α1 interacts closely with other interferons and cytokines to enhance antiviral states. Through these pathways it shares functional relationships with proteins such as STAT1 and IRF7 which are critical for mounting effective antiviral defenses.

Interferon alpha 1 relates closely to conditions like hepatitis C and multiple sclerosis. It has therapeutic applications in these diseases due to its immunoregulatory effects. IFN-α1 interacts with various immune system components including major histocompatibility complex (MHC) proteins to modulate immune responses. Abnormalities in IFN-α1 signaling or expression can exacerbate inflammatory and autoimmune disorders linking it to both protective and pathological roles in human health.
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Specifications

Form

Lyophilized

Additional notes

Purity by HPLC = 95%

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General info

Function

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.

Sequence similarities

Belongs to the type-I (or alpha/beta) interferon family.

Post-translational modifications

Glycosylated.

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Product protocols

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Target data

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.
See full target information Ifna1
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