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AB310803

Recombinant Mouse Klotho protein

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Recombinant Mouse Klotho protein is a Mouse Fragment protein, in the 35 to 550 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE.

View Alternative Names

Klotho, Kl

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Mass Spectrometry - Recombinant Mouse Klotho protein (AB310803)
  • Mass Spec

Lab

Mass Spectrometry - Recombinant Mouse Klotho protein (AB310803)

Mass determination by ESI-TOF. Predicted MW is 58652.62668 (+/- 10Da by ESI-TOF). Observed coverage is 62.09% via TPP.

SDS-PAGE - Recombinant Mouse Klotho protein (AB310803)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse Klotho protein (AB310803)

SDS-PAGE analysis of ab310803

HPLC - Recombinant Mouse Klotho protein (AB310803)
  • HPLC

Lab

HPLC - Recombinant Mouse Klotho protein (AB310803)

HPLC analysis of ab310803

SDS-PAGE - Recombinant Mouse Klotho protein (AB310803)
  • SDS-PAGE

Lab

SDS-PAGE - Recombinant Mouse Klotho protein (AB310803)

SDS-PAGE analysis of ab310803

Key facts

Purity

>95% HPLC

SDS-PAGE >= 95%

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

HPLC, Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

O35082

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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Primary Antibodies

AB203576

Anti-Klotho antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody (AB203576)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Bioactive

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"AEPGQGAQTWARFARAPAPEAAGLLHDTFPDGFLWAVGSAAYQTEGGWRQHGKGASIWDTFTHHSGAAPSDSPIVVAPSGAPSPPLSSTGDVASDSYNNVYRDTEGLRELGVTHYRFSISWARVLPNGTAGTPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDTYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGVRGSSRLGYLVAHNLLLAHAKVWHLYNTSFRPTQGGRVSIALSSHWINPRRMTDYNIRECQKSLDFVLGWFAKPIFIDGDYPESMKNNLSSLLPDFTESEKRLIRGTADFFALSFGPTLSFQLLDPNMKFRQLESPNLRQLLSWIDLEYNHPPIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIRLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKELLPKSSALFYQKLIEDNGFPPLPENQPLEGTFPCDFAWGVVDNYVQVDTTLSQFTDPNVYL","proteinLength":"Fragment","predictedMolecularWeight":"58.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":550,"aminoAcidStart":35,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"O35082","tags":[]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Klotho also known as alpha Klotho protein is a multifunctional protein prominently involved in regulating aging processes. It consists of approximately 130 kDa and is expressed mainly in the kidneys brain and parathyroid gland. This membrane-bound protein may also exist in a soluble form after cleavage by specific enzymes. Alpha Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) facilitating phosphate and vitamin D metabolism regulation.
Biological function summary

Alpha Klotho influences several cellular processes such as aging and mineral ion homeostasis. As a part of the FGF23-Klotho complex it enables the fibroblast growth factor to exert its biological effects. This protein modulates the effects of FGF23 on the renal excretion of phosphate and the synthesis of vitamin D. By interacting with FGF23 alpha Klotho supports maintaining electrolyte balance vital for proper physiological functioning.

Pathways

The FGF23-Klotho axis integrates into significant signaling pathways regulating mineral metabolism and aging. Klotho protein interactions with FGF23 contribute to controlling phosphate homeostasis by inhibiting renal phosphate reabsorption and suppressing vitamin D synthesis. It also engages with other proteins notably the fibroblast growth factors to regulate aging-related pathways ensuring cellular longevity and repair mechanisms are optimized.

Understanding Klotho's role helps in managing conditions like chronic kidney disease and some neurological disorders. Research indicates that reduced levels of alpha Klotho may correspond to kidney dysfunction due to its critical role in mineral metabolism. Furthermore alterations in Klotho expression connect with neurodegenerative diseases implicating its interaction with other proteins such as amyloid precursor protein and tau which are significant in disorders like Alzheimer's disease.
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General info

Function

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
See full target information Kl
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