Recombinant Mouse Lactoferrin protein (His tag) is a Mouse Full Length protein, in the 1 to 707 aa range, expressed in HEK 293, with >92% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >92% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M R L L I P S L I F L E A L G L C L A K A T T V Q W C A V S N S E E E K C L R W Q N E M R K V G G P P L S C V K K S S T R Q C I Q A I V T N R A D A M T L D G G T M F D A G K P P Y K L R P V A A E V Y G T K E Q P R T H Y Y A V A V V K N S S N F H L N Q L Q G L R S C H T G I G R S A G W K I P I G T L R P Y L N W N G P P A S L E E A V S K F F S K S C V P G A Q K D R F P N L C S S C A G T G A N K C A S S P E E P Y S G Y A G A L R C L R D N A G D V A F T R G S T V F E E L P N K A E R D Q Y K L L C P D N T W K P V T E Y K E C H L A Q V P S H A V V S R S T N D K E E A I W E L L R Q S Q E K F G K K Q A S G F Q L F A S P S G Q K D L L F K E S A I G F V R V P Q K V D V G L Y L T F S Y T T S I Q N L N K K Q Q D V I A S K A R V T W C A V G S E E K R K C D Q W N R A S R G R V T C I S F P T T E D C I V A I M K G D A D A M S L D G G Y I Y T A G K C G L V P V L A E N Q K S S K S N G L D C V N R P V E G Y L A V A A V R R E D A G F T W S S L R G K K S C H T A V D R T A G W N I P M G L L A N Q T R S C K F N E F F S Q S C A P G A D P K S N L C A L C I G D E K G E N K C A P N S K E R Y Q G Y T G A L R C L A E K A G N V A F L K D S T V L Q N T D G K N T E E W A R N L K L K D F E L L C L D D T R K P V T E A K N C H L A I A P N H A V V S R T D K V E V L Q Q V L L D Q Q V Q F G R N G Q R C P G E F C L F Q S K T K N L L F N D N T E C L A K I P G K T T S E K Y L G K E Y V I A T E R L K Q C S S S P L L E A C A F L T Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin. Major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity, which depends on the extracellular cation concentration. Antimicrobial properties include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. Can also prevent bacterial biofilm development in P.aeruginosa infection. Has weak antifungal activity against C.albicans. Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. Promotes binding of species C adenoviruses to epithelial cells, promoting adenovirus infection. Can inhibit papillomavirus infections. Stimulates the TLR4 signaling pathway leading to NF-kappa-B activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling. Inhibits neutrophil granulocyte migration to sites of apoptosis, when secreted by apoptotic cells. Stimulates VEGFA-mediated endothelial cell migration and proliferation. Binds heparin, chondroitin sulfate and possibly other glycosaminoglycans (GAGs). Also binds specifically to pneumococcal surface protein A (PspA), the lipid A portion of bacterial lipopolysaccharide (LPS), lysozyme and DNA. Lactoferricin binds to the bacterial surface and is crucial for the bactericidal functions. Has some antiviral activity against papillomavirus infection. N-terminal region shows strong antifungal activity against C.albicans. Contains two BBXB heparin-binding consensus sequences that appear to form the predominate functional GAG-binding site. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites.
Alternative names
Lactotransferrin, Lactoferrin, Ltf
Recommended products
Recombinant Mouse Lactoferrin protein (His tag) is a Mouse Full Length protein, in the 1 to 707 aa range, expressed in HEK 293, with >92% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >92% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P08071-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M R L L I P S L I F L E A L G L C L A K A T T V Q W C A V S N S E E E K C L R W Q N E M R K V G G P P L S C V K K S S T R Q C I Q A I V T N R A D A M T L D G G T M F D A G K P P Y K L R P V A A E V Y G T K E Q P R T H Y Y A V A V V K N S S N F H L N Q L Q G L R S C H T G I G R S A G W K I P I G T L R P Y L N W N G P P A S L E E A V S K F F S K S C V P G A Q K D R F P N L C S S C A G T G A N K C A S S P E E P Y S G Y A G A L R C L R D N A G D V A F T R G S T V F E E L P N K A E R D Q Y K L L C P D N T W K P V T E Y K E C H L A Q V P S H A V V S R S T N D K E E A I W E L L R Q S Q E K F G K K Q A S G F Q L F A S P S G Q K D L L F K E S A I G F V R V P Q K V D V G L Y L T F S Y T T S I Q N L N K K Q Q D V I A S K A R V T W C A V G S E E K R K C D Q W N R A S R G R V T C I S F P T T E D C I V A I M K G D A D A M S L D G G Y I Y T A G K C G L V P V L A E N Q K S S K S N G L D C V N R P V E G Y L A V A A V R R E D A G F T W S S L R G K K S C H T A V D R T A G W N I P M G L L A N Q T R S C K F N E F F S Q S C A P G A D P K S N L C A L C I G D E K G E N K C A P N S K E R Y Q G Y T G A L R C L A E K A G N V A F L K D S T V L Q N T D G K N T E E W A R N L K L K D F E L L C L D D T R K P V T E A K N C H L A I A P N H A V V S R T D K V E V L Q Q V L L D Q Q V Q F G R N G Q R C P G E F C L F Q S K T K N L L F N D N T E C L A K I P G K T T S E K Y L G K E Y V I A T E R L K Q C S S S P L L E A C A F L T Q
- Accession
- P08071
- Protein length
- Full Length
- Predicted molecular weight
- 77.2 kDa
- Amino acids
- 1 to 707
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
- Sequence similarities
Belongs to the transferrin family.
- Post-translational modifications
Poly-N-acetyllactosaminic carbohydrate moiety seems to be needed for TLR4 activation.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lactoferrin also known as lactotransferrin is an iron-binding glycoprotein with a molecular weight of about 80 kDa. It predominantly exists in mammalian milk particularly in high concentrations in colostrum and is also found in various epithelial secretions such as saliva tears and nasal fluids. The liver and neutrophils also produce lactoferrin and it plays a significant role in the body's primary defense system. As an antibacterial agent lactoferrin sequesters free iron to limit bacterial growth and disrupt biofilms assisting in the protection against microbial invasions.
- Biological function summary
Lactoferrin exhibits multiple functions beyond its antimicrobial properties. It contributes to immunomodulation by binding to specific receptors on immune cells enhancing phagocytosis and influencing cytokine production. Also it possesses anti-inflammatory and antioxidant properties. Lactoferrin interacts with cellular components including glycosaminoglycans and lipoproteins impacting the activity and expression of proteins within signaling and metabolic pathways. This multifaceted role places it as a critical participant in both innate and adaptive immune responses although it does not form part of a stable protein complex.
- Pathways
Lactoferrin plays a significant role in the iron metabolism and immune response pathways. It tightly interacts with the transferrin receptor modulating iron uptake and distribution across tissues which helps prevent iron overload and oxidative stress. Furthermore lactoferrin links with the toll-like receptor pathways particularly TLR4 which enables the modulation of innate immune responses against pathogens. By influencing nuclear factor kappa B (NF-kB) signaling lactoferrin impacts inflammation and immune responses working together with proteins such as ferritin and hepcidin.
- Associated diseases and disorders
Lactoferrin has relevancy to diseases like anemia of chronic disease and inflammatory bowel disease (IBD). Its role in regulating iron homeostasis makes it essential for preventing anemia associated with chronic inflammation. Lactoferrin mitigates dysregulated immune responses in IBD potentially reducing inflammation and promoting gut health. Disease-modifying properties of lactoferrin connect it to glycoproteins like transferrin and inflammatory mediators demonstrating its potential therapeutic importance.
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SDS-PAGE - Recombinant Mouse Lactoferrin protein (His tag) (ab276922)
SDS-PAGE analysis of ab276922
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