Recombinant Mouse LAG-3 protein (Tagged) is a Mouse Fragment protein, in the 23 to 442 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>=90% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
Avi tag C-Terminus
Fc tag C-Terminus- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
S G P G K E L P V V W A Q E G A P V H L P C S L K S P N L D P N F L R R G G V I W Q H Q P D S G Q P T P I P A L D L H Q G M P S P R Q P A P G R Y T V L S V A P G G L R S G R Q P L H P H V Q L E E R G L Q R G D F S L W L R P A L R T D A G E Y H A T V R L P N R A L S C S L R L R V G Q A S M I A S P S G V L K L S D W V L L N C S F S R P D R P V S V H W F Q G Q N R V P V Y N S P R H F L A E T F L L L P Q V S P L D S G T W G C V L T Y R D G F N V S I T Y N L K V L G L E P V A P L T V Y A A E G S R V E L P C H L P P G V G T P S L L I A K W T P P G G G P E L P V A G K S G N F T L H L E A V G L A Q A G T Y T C S I H L Q G Q Q L N A T V T L A V I T V T P K S F G L P G S R G K L L C E V T P A S G K E R F V W R P L N N L S R S C P G P V L E I Q E A R L L A E R W Q C Q L Y E G Q R L L G A T V Y A A E S S S G A H S A R R I S G D L K G G H L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887, PubMed:30580966). Delivers inhibitory signals upon binding to ligands, such as FGL1 (PubMed:30580966). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (PubMed:30580966). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (PubMed:21300912). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (PubMed:15485628). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (PubMed:19201841). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:12209638, PubMed:12421911, PubMed:15634887).Secreted lymphocyte activation gene 3 proteinMay function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.
Alternative names
CD223, Lymphocyte activation gene 3 protein, LAG-3, Activation-induced cytidine deaminase-linked autoimmunity protein, Aida, Lag3
Recommended products
Recombinant Mouse LAG-3 protein (Tagged) is a Mouse Fragment protein, in the 23 to 442 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>=90% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
Avi tag C-Terminus
Fc tag C-Terminus- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q61790-1
- Animal free
No
- Species
Mouse
- Concentration
- Storage buffer
pH: 5.5
Constituents: 10% Glycerol (glycerin, glycerine), 2.9% Sodium chloride, 0.16% Sodium acetate, 0.02% Sorbitan monolaurate, ethoxylated
Sequence info
Amino acid sequence
S G P G K E L P V V W A Q E G A P V H L P C S L K S P N L D P N F L R R G G V I W Q H Q P D S G Q P T P I P A L D L H Q G M P S P R Q P A P G R Y T V L S V A P G G L R S G R Q P L H P H V Q L E E R G L Q R G D F S L W L R P A L R T D A G E Y H A T V R L P N R A L S C S L R L R V G Q A S M I A S P S G V L K L S D W V L L N C S F S R P D R P V S V H W F Q G Q N R V P V Y N S P R H F L A E T F L L L P Q V S P L D S G T W G C V L T Y R D G F N V S I T Y N L K V L G L E P V A P L T V Y A A E G S R V E L P C H L P P G V G T P S L L I A K W T P P G G G P E L P V A G K S G N F T L H L E A V G L A Q A G T Y T C S I H L Q G Q Q L N A T V T L A V I T V T P K S F G L P G S R G K L L C E V T P A S G K E R F V W R P L N N L S R S C P G P V L E I Q E A R L L A E R W Q C Q L Y E G Q R L L G A T V Y A A E S S S G A H S A R R I S G D L K G G H L
- Accession
- Q61790
- Protein length
Fragment
- Predicted molecular weight
74 kDa
- Amino acids
23 to 442
- Nature
Recombinant
- Tags
Avi tag C-Terminus, Fc tag C-Terminus
Specifications
- Form
Liquid
General info
- Function
Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887, PubMed:30580966). Delivers inhibitory signals upon binding to ligands, such as FGL1 (PubMed:30580966). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (PubMed:30580966). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (PubMed:21300912). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (PubMed:15485628). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (PubMed:19201841). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:12209638, PubMed:12421911, PubMed:15634887).
- Sequence similarities
Belongs to the LAG3 family.
- Post-translational modifications
Lymphocyte activation gene 3 protein
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LAG-3 also known as CD223 is a transmembrane protein with a mass of approximately 70 kDa. It acts as a checkpoint molecule similar to CTLA-4 and PD-1 in the immune system. LAG-3 is expressed on activated T cells natural killer cells and some dendritic cells. It interacts with its ligand MHC class II leading to inhibition of cellular proliferation and cytokine secretion an important part of immune response regulation.
- Biological function summary
LAG-3 is involved in maintaining immune homeostasis and preventing autoimmunity. It is not part of a complex but works in synergy with other immune checkpoint molecules. When interacting with MHC class II LAG-3 transmits inhibitory signals that attenuate immune cell responses. Its role in immune regulation helps to balance immune activation and tolerance which is essential for preventing tissue damage while protecting against pathogens.
- Pathways
The inhibitory role of LAG-3 integrates into the T cell receptor (TCR) signaling pathway and is also linked to the PD-1 signaling pathway. In TCR signaling LAG-3 negatively regulates T cell activation. LAG-3 is functionally related to proteins like PD-1 and CTLA-4 sharing similar roles in immune checkpoint pathways that are critical for maintaining immune balance.
- Associated diseases and disorders
LAG-3 has links to cancer and autoimmune diseases. In cancer LAG-3 expression on tumor-infiltrating lymphocytes correlates with immune evasion by tumors. This relationship appears in various cancers including melanoma and renal cell carcinoma. In autoimmune diseases LAG-3's expression on regulatory T cells helps control conditions such as multiple sclerosis. It collaborates with CTLA-4 in modulating immune responses that prevent excessive immune activation and tissue damage associated with autoimmunity.
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SDS-PAGE - Recombinant Mouse LAG-3 protein (Tagged) (ab271582)
SDS-PAGE analysis of 3 μg ab271582.
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