Recombinant Mouse Legumain protein (His tag) is a Mouse Full Length protein, in the 18 to 325 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
V P V G V D D P E D G G K H W V V I V A G S N G W Y N Y R H Q A D A C H A Y Q I I H R N G I P D E Q I I V M M Y D D I A N S E E N P T P G V V I N R P N G T D V Y K G V L K D Y T G E D V T P E N F L A V L R G D A E A V K G K G S G K V L K S G P R D H V F I Y F T D H G A T G I L V F P N D D L H V K D L N K T I R Y M Y E H K M Y Q K M V F Y I E A C E S G S M M N H L P D D I N V Y A T T A A N P K E S S Y A C Y Y D E E R G T Y L G D W Y S V N W M E D S D V E D L T K E T L H K Q Y H L V K S H T N T S H V M Q Y G N K S I S T M K V M Q F Q G M K H R A S S P I S L P P V T H L D L T P S P D V P L T I L K R K L L R T N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed:24407422, PubMed:9742219, PubMed:9891971). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed:24407422, PubMed:9742219, PubMed:9891971). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (By similarity). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (PubMed:37347855). Required for normal lysosomal protein degradation in renal proximal tubules (PubMed:17350006, PubMed:21292981). Required for normal degradation of internalized EGFR (PubMed:21292981). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (PubMed:21292981).
Alternative names
Prsc1, Lgmn, Legumain
Recommended products
Recombinant Mouse Legumain protein (His tag) is a Mouse Full Length protein, in the 18 to 325 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O89017-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- V P V G V D D P E D G G K H W V V I V A G S N G W Y N Y R H Q A D A C H A Y Q I I H R N G I P D E Q I I V M M Y D D I A N S E E N P T P G V V I N R P N G T D V Y K G V L K D Y T G E D V T P E N F L A V L R G D A E A V K G K G S G K V L K S G P R D H V F I Y F T D H G A T G I L V F P N D D L H V K D L N K T I R Y M Y E H K M Y Q K M V F Y I E A C E S G S M M N H L P D D I N V Y A T T A A N P K E S S Y A C Y Y D E E R G T Y L G D W Y S V N W M E D S D V E D L T K E T L H K Q Y H L V K S H T N T S H V M Q Y G N K S I S T M K V M Q F Q G M K H R A S S P I S L P P V T H L D L T P S P D V P L T I L K R K L L R T N
- Accession
- O89017
- Protein length
- Full Length
- Predicted molecular weight
- 38.8 kDa
- Amino acids
- 18 to 325
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed:24407422, PubMed:9742219, PubMed:9891971). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed:24407422, PubMed:9742219, PubMed:9891971). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (By similarity). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (PubMed:37347855). Required for normal lysosomal protein degradation in renal proximal tubules (PubMed:17350006, PubMed:21292981). Required for normal degradation of internalized EGFR (PubMed:21292981). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (PubMed:21292981).
- Sequence similarities
Belongs to the peptidase C13 family.
- Post-translational modifications
Glycosylated.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Legumain also known as asparaginyl endopeptidase is an enzyme that plays a mechanical role as a protease. This enzyme has a molecular mass of approximately 56 kDa and it functions by catalyzing the hydrolysis of asparagine residues. Legumain is expressed in various tissues with higher levels observed in kidneys placenta and liver. Its proteolytic activity is essential for the breakdown of intracellular proteins within lysosomes and endosomal compartments.
- Biological function summary
Legumain contributes to protein degradation and antigen processing. It does not operate alone as it is part of larger protein complexes which enhance its stability and function. Through its role in proteolysis legumain influences cellular processes like maturation of endolysosomal enzymes and processing of proenzymes. Additionally it assists in activating other proteases signifying its critical position in maintaining cellular proteostasis.
- Pathways
Legumain participates in the regulation of immune responses and cellular homeostasis. It plays a significant role in the lysosomal pathway where it partners with cathepsins to assist in the degradation of proteins. Additionally legumain influences the MHC class II pathway by contributing to the cleavage of invariant chain aiding antigen presentation. Its interaction with cathepsins and the impact on antigen processing indicates its necessity in immune system functionality.
- Associated diseases and disorders
Legumain has been linked to disorders such as cancer and neurodegeneration. In cancer elevated legumain levels correlate with tumor invasiveness and metastasis especially in association with proteins like matrix metalloproteinases. Meanwhile in neurodegenerative conditions its dysregulation may contribute to abnormal protein accumulation and synaptic dysfunction. Understanding legumain's role and interactions can guide therapeutic strategies for these diseases.
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1 product image
SDS-PAGE - Recombinant Mouse Legumain protein (His tag) (ab235559)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel of ab235559.
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