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AB282399

Recombinant Mouse Lipocalin-2 / NGAL protein (Active)

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Recombinant Mouse Lipocalin-2 / NGAL protein (Active) is a Mouse Full Length protein, in the 21 to 200 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, FuncS.

View Alternative Names

Neutrophil gelatinase-associated lipocalin, NGAL, Lipocalin-2, Oncogene 24p3, SV-40-induced 24p3 protein, Siderocalin LCN2, p25, 24p3, Lcn2

4 Images
Functional Studies - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399)
  • FuncS

Supplier Data

Functional Studies - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399)

Fully biologically active determined by its ability to bind Iron(III) dihydroxybenzoic acid [Fe3+ (DHBA)3] results in the quenching of Trp fluorescence in Lipocalin-2. Recombinant Mouse LCN2 can bind ≥ 3.3 uM [Fe3+ (DHBA)3].

Mass Spectrometry - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399)

Mass determination by ESI-TOF.

Predicted MW is 20931.55 Da (+/- 10 Da by ESI-TOF). Observed MW is 20933.81

SDS-PAGE - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399)

SDS-page analysis of ab282399

HPLC - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399)
  • HPLC

Supplier Data

HPLC - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399)

HPLC analysis of ab282399

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, HPLC, Mass Spec

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by its ability to bind Iron(III) dihydroxybenzoic acid [Fe3+ (DHBA)3] results in the quenching of Trp fluorescence in Lipocalin-2.

Recombinant Mouse LCN2 can bind ≥ 3.3 uM [Fe3+ (DHBA)3].

Accession

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"QDSTQNLIPAPSLLTVPLQPDFRSDQFRGRWYVVGLAGNAVQKKTEGSFTMYSTIYELQENNSYNVTSILVRDQDQGCRYWIRTFVPSSRAGQFTLGNMHRYPQVQSYNVQVATTDYNQFAMVFFRKTSENKQYFKITLYGRTKELSPELKERFTRFAKSLGLKDDNIIFSVPTDQCIDN","proteinLength":"Full Length","predictedMolecularWeight":"20.93 kDa","actualMolecularWeight":null,"aminoAcidEnd":200,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P11672","tags":[]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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General info

Function

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed : 12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation : iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed : 15531878, PubMed : 16446425). Can also bind siderophores from M.tuberculosis (By similarity).

Sequence similarities

Belongs to the calycin superfamily. Lipocalin family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed : 12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation : iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed : 15531878, PubMed : 16446425). Can also bind siderophores from M.tuberculosis (By similarity).
See full target information Lcn2
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