Recombinant Mouse Lipocalin-2 / NGAL protein (Active) is a Mouse Full Length protein, in the 21 to 200 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
>=95% HPLC
<= 0.005 EU/µg
HEK 293 cells
Tag free
SDS-PAGE, MS, HPLC
Yes
Q D S T Q N L I P A P S L L T V P L Q P D F R S D Q F R G R W Y V V G L A G N A V Q K K T E G S F T M Y S T I Y E L Q E N N S Y N V T S I L V R D Q D Q G C R Y W I R T F V P S S R A G Q F T L G N M H R Y P Q V Q S Y N V Q V A T T D Y N Q F A M V F F R K T S E N K Q Y F K I T L Y G R T K E L S P E L K E R F T R F A K S L G L K D D N I I F S V P T D Q C I D N
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:15531878, PubMed:16446425). Can also bind siderophores from M.tuberculosis (By similarity).
Neutrophil gelatinase-associated lipocalin, NGAL, Lipocalin-2, Oncogene 24p3, SV-40-induced 24p3 protein, Siderocalin LCN2, p25, 24p3, Lcn2
Recombinant Mouse Lipocalin-2 / NGAL protein (Active) is a Mouse Full Length protein, in the 21 to 200 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
>=95% HPLC
<= 0.005 EU/µg
HEK 293 cells
Tag free
SDS-PAGE, MS, HPLC
Yes
Fully biologically active determined by its ability to bind Iron(III) dihydroxybenzoic acid [Fe3+ (DHBA)3] results in the quenching of Trp fluorescence in Lipocalin-2.
Recombinant Mouse LCN2 can bind ≥ 3.3 uM [Fe3+ (DHBA)3].
Yes
Mouse
Reconstitute in PBS
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Q D S T Q N L I P A P S L L T V P L Q P D F R S D Q F R G R W Y V V G L A G N A V Q K K T E G S F T M Y S T I Y E L Q E N N S Y N V T S I L V R D Q D Q G C R Y W I R T F V P S S R A G Q F T L G N M H R Y P Q V Q S Y N V Q V A T T D Y N Q F A M V F F R K T S E N K Q Y F K I T L Y G R T K E L S P E L K E R F T R F A K S L G L K D D N I I F S V P T D Q C I D N
Full Length
20.93 kDa
21 to 200
Recombinant
Lyophilized
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:15531878, PubMed:16446425). Can also bind siderophores from M.tuberculosis (By similarity).
Belongs to the calycin superfamily. Lipocalin family.
N-glycosylated.
Ambient - Can Ship with Ice
Ambient
Ambient
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SDS-page analysis of ab282399
Mass determination by ESI-TOF.
Predicted MW is 20931.55 Da (+/- 10 Da by ESI-TOF). Observed MW is 20933.81
HPLC analysis of ab282399
Fully biologically active determined by its ability to bind Iron(III) dihydroxybenzoic acid [Fe3+ (DHBA)3] results in the quenching of Trp fluorescence in Lipocalin-2. Recombinant Mouse LCN2 can bind ≥ 3.3 uM [Fe3+ (DHBA)3].
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