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Recombinant Mouse Lipocalin-2 / NGAL protein (Active) is a Mouse Full Length protein, in the 21 to 200 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.

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Images

SDS-PAGE - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399), expandable thumbnail
  • Mass Spectrometry - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399), expandable thumbnail
  • HPLC - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399), expandable thumbnail
  • Biological Activity - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (AB282399), expandable thumbnail

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, MS, HPLC

Biologically active

Yes

Amino acid sequence

Q D S T Q N L I P A P S L L T V P L Q P D F R S D Q F R G R W Y V V G L A G N A V Q K K T E G S F T M Y S T I Y E L Q E N N S Y N V T S I L V R D Q D Q G C R Y W I R T F V P S S R A G Q F T L G N M H R Y P Q V Q S Y N V Q V A T T D Y N Q F A M V F F R K T S E N K Q Y F K I T L Y G R T K E L S P E L K E R F T R F A K S L G L K D D N I I F S V P T D Q C I D N

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:15531878, PubMed:16446425). Can also bind siderophores from M.tuberculosis (By similarity).

Alternative names

Recommended products

Recombinant Mouse Lipocalin-2 / NGAL protein (Active) is a Mouse Full Length protein, in the 21 to 200 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, MS, HPLC

Biological activity

Fully biologically active determined by its ability to bind Iron(III) dihydroxybenzoic acid [Fe3+ (DHBA)3] results in the quenching of Trp fluorescence in Lipocalin-2.

Recombinant Mouse LCN2 can bind ≥ 3.3 uM [Fe3+ (DHBA)3].

Accession
P11672-1
Animal free

Yes

Species

Mouse

Reconstitution

Reconstitute in PBS

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Q D S T Q N L I P A P S L L T V P L Q P D F R S D Q F R G R W Y V V G L A G N A V Q K K T E G S F T M Y S T I Y E L Q E N N S Y N V T S I L V R D Q D Q G C R Y W I R T F V P S S R A G Q F T L G N M H R Y P Q V Q S Y N V Q V A T T D Y N Q F A M V F F R K T S E N K Q Y F K I T L Y G R T K E L S P E L K E R F T R F A K S L G L K D D N I I F S V P T D Q C I D N

Accession

P11672

Protein length

Full Length

Predicted molecular weight

20.93 kDa

Amino acids

21 to 200

Nature

Recombinant

Specifications

Form

Lyophilized

General info

Function

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:15531878, PubMed:16446425). Can also bind siderophores from M.tuberculosis (By similarity).

Sequence similarities

Belongs to the calycin superfamily. Lipocalin family.

Post-translational modifications

N-glycosylated.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • SDS-PAGE - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (ab282399), expandable thumbnail

    SDS-PAGE - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (ab282399)

    SDS-page analysis of ab282399

  • Mass Spectrometry - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (ab282399), expandable thumbnail

    Mass Spectrometry - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (ab282399)

    Mass determination by ESI-TOF.

    Predicted MW is 20931.55 Da (+/- 10 Da by ESI-TOF). Observed MW is 20933.81

  • HPLC - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (ab282399), expandable thumbnail

    HPLC - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (ab282399)

    HPLC analysis of ab282399

  • Biological Activity - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (ab282399), expandable thumbnail

    Biological Activity - Recombinant Mouse Lipocalin-2 / NGAL protein (Active) (ab282399)

    Fully biologically active determined by its ability to bind Iron(III) dihydroxybenzoic acid [Fe3+ (DHBA)3] results in the quenching of Trp fluorescence in Lipocalin-2. Recombinant Mouse LCN2 can bind ≥ 3.3 uM [Fe3+ (DHBA)3]. 

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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