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AB129146

Recombinant mouse M-CSF protein

5

(1 Review)

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(7 Publications)

Recombinant mouse M-CSF protein is a Mouse Fragment protein, in the 33 to 187 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Csfm, Csf1, Macrophage colony-stimulating factor 1, CSF-1, MCSF, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF

2 Images
Functional Studies - Recombinant mouse M-CSF protein (AB129146)
  • FuncS

Unknown

Functional Studies - Recombinant mouse M-CSF protein (AB129146)

Functional analysis of ab129146

Pulse Labelling - Recombinant mouse M-CSF protein (AB129146)
  • Pulse Labelling

Supplier Data

Pulse Labelling - Recombinant mouse M-CSF protein (AB129146)

1 ug of ab129146 on 4-20% Tris-Glycine SDS-PAGE, stained with Coomassie Blue.

Lane 1 : Non-reducing

Lane 2 : Reducing.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Method of Determination: NFS-60 cell proliferation

Acception Criteria: ED50 ≤10 ng/mL(≥ 1.0 x 10^5 units/mg)

Accession

P07141

Animal free

No

Carrier free

Yes

Species

Mouse

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.29% Sodium chloride, 0.16% Sodium phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Endotoxin levels determined by kinetic LAL (50% Confidence).
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Sequence info

[{"sequence":"MKEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFVDQEQLDDPVCYLKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQNKACVRTFHETPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSRDVVTKPMKEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFVDQEQLDDPVCYLKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQNKACVRTFHETPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSRDVVTKP","proteinLength":"Fragment","predictedMolecularWeight":"36.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":187,"aminoAcidStart":33,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P07141","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon reconstitution add a carrier protein (0.1% BSA)
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.
Biological function summary

M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.

Pathways

The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.

Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.
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Specifications

Form

Lyophilized

Additional notes

Purified using standard chromatographical techniques.

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General info

Function

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

Post-translational modifications

N-glycosylated.. O-glycosylated; contains chondroitin sulfate.

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Product protocols

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Target data

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
See full target information Csf1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 12:1509136 PubMed39624773

2024

Fe-capsaicin nanozyme attenuates sepsis-induced acute lung injury by regulating the functions of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijie Wang,Ning Zhou,Jinfang Xue,Yan Li,Yang Wang,Huadong Zhu,Chuanzhu Lv

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2407662 PubMed39563492

2024

Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xinwen Wang,Min Wang,Qijun Lin,Lixin He,Baolin Zhang,Xin Chen,Guanhong Chen,Hong Du,Chuandong Lang,Xinsheng Peng,Yuhu Dai

Journal of inflammation research 15:3337-3353 PubMed35702548

2022

Nociceptor Neurons are Involved in the Host Response to Urinary Tract Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengdong Gao,Yaxiao Liu,Lekai Zhang,Zizhuo Yang,Linchen Lv,Shuai Wang,Lipeng Chen,Nan Zhou,Yaofeng Zhu,Xuewen Jiang,Benkang Shi,Yan Li

The Journal of biological chemistry 298:101711 PubMed35150745

2022

SIRT6 stabilization and cytoplasmic localization in macrophages regulates acute and chronic inflammation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana Bresque,Karina Cal,Valentina Pérez-Torrado,Laura Colman,Jorge Rodríguez-Duarte,Cecilia Vilaseca,Leonardo Santos,María Pía Garat,Santiago Ruiz,Frances Evans,Rosina Dapueto,Paola Contreras,Aldo Calliari,Carlos Escande

Frontiers in pharmacology 12:816032 PubMed35115947

2022

Restrains Hepatic Pro-Inflammatory Macrophages to Ameliorate Non-Alcoholic Fatty Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyuan Zheng,Dan Fang,Chaoyuan Huang,Lina Zhao,Liming Gan,Youlan Chen,Fengbin Liu

Journal of inflammation research 14:1973-1989 PubMed34045879

2021

Urinary Trypsin Inhibitor Protects Tight Junctions of Septic Pulmonary Capillary Endothelial Cells by Regulating the Functions of Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijie Wang,Wenliang Song,Chengyuan Xie,Wenhong Zhong,Hui Xu,Qiuping Zhou,Yiyu Deng,Yimei Hong,Xin Li,Ming Fang

Journal of orthopaedic translation 27:9-16 PubMed33344167

2020

Hepatocyte growth factor overexpression promotes osteoclastogenesis and exacerbates bone loss in CIA mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoming Huang,Yufan Zheng,Jinyu Bai,Ce Shi,Xin Shi,Huajian Shan,Xiaozhong Zhou
View all publications
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