Recombinant Mouse Macrophage Inflammatory Protein 1 alpha / CCL3 is a Mouse Full Length protein, in the 24 to 92 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
A P Y G A D T P T A C C F S Y S R K I P R Q F I V D Y F E T S S L C S Q P G V I F L T K R N R Q I C A D S K E T W V Q E Y I T D L E L N A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Alternative names
Mip1a, Scya3, Ccl3, C-C motif chemokine 3, Heparin-binding chemotaxis protein, L2G25B, Macrophage inflammatory protein 1-alpha, SIS-alpha, Small-inducible cytokine A3, TY-5, MIP-1-alpha
Recommended products
Recombinant Mouse Macrophage Inflammatory Protein 1 alpha / CCL3 is a Mouse Full Length protein, in the 24 to 92 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- No
- Accession
- P10855-1
- Animal free
- No
- Species
- Mouse
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A P Y G A D T P T A C C F S Y S R K I P R Q F I V D Y F E T S S L C S Q P G V I F L T K R N R Q I C A D S K E T W V Q E Y I T D L E L N A
- Accession
- P10855
- Protein length
- Full Length
- Predicted molecular weight
- 7.9 kDa
- Actual molecular weight
- 7.9 kDa
- Amino acids
- 24 to 92
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
- Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL3L1 also known as chemokine (C-C motif) ligand 3-like 1 is a protein encoded by the CCL3L1 gene. This chemokine weighs approximately 10 kDa. CCL3L1 binds to chemokine receptors CCR1 and CCR5 on the surface of target cells. It is secreted by various cells such as macrophages T-cells and dendritic cells. Mainly it expresses in tissues involved in immune responses including lymph nodes and spleen.
- Biological function summary
CCL3L1 plays an important role in mediating immune responses. It attracts immune cells like monocytes neutrophils and T-lymphocytes to sites of infection or injury enhancing the immune defense. Although CCL3L1 does not form part of a larger protein complex it acts as a critical signaling molecule in the immune system. Its interaction with CCR5 makes it an important component in inflammatory responses and leukocyte chemotaxis.
- Pathways
CCL3L1 functions as a significant participant in cytokine-cytokine receptor interactions and chemokine signaling pathways. These pathways regulate the movement and activity of immune cells in response to specific chemokine signals. Through these pathways CCL3L1 interacts with other chemokines such as CCL4 supporting the coordination of immune cell trafficking to inflamed tissues. This involvement is essential for maintaining the proper function of the immune response.
- Associated diseases and disorders
CCL3L1 is associated with susceptibility to HIV infection and rheumatoid arthritis. Variations in the CCL3L1 gene copy number can alter individual risk to HIV as this protein competes for binding with the HIV-1 virus on the CCR5 receptor effectively impeding viral entry. Additionally the overexpression of CCL3L1 contributes to the inflammatory environment observed in rheumatoid arthritis by recruiting more immune cells to affected joints and it interacts with proteins like TNF-α in the inflammatory cascade.
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HPLC - Recombinant Mouse Macrophage Inflammatory Protein 1 alpha / CCL3 (ab310785)
HPLC analysis of ab310785
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