Recombinant mouse MAP3K8/COT protein (His tag) is a Mouse Fragment protein, in the 30 to 397 aa range, expressed in Baculovirus infected Sf9, with >=38% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=38% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M E N L Y A S E E P G V Y E P S L M T M Y P D S N Q N E E R S E S L L R S G Q E V P W L S S V R Y G T V E D L L A F A N H V S N M T K H F Y G R R P Q E C G I L L N M V I S P Q N G R Y Q I D S D V L L V P W K L T Y R N I G S G F V P R G A F G K V Y L A Q D M K T K K R M A C K L I P I D Q F K P S D V E I Q A C F R H E N I A E L Y G A V L W G D T V H L F M E A G E G G S V L E K L E S C G P M R E F E I I W V T K H I L K G L D F L H S K K V I H H D I K P S N I V F M S T K A V L V D F G L S V K M T E D V Y L P K D L R G T E I Y M S P E V I L C R G H S T K A D I Y S L G A T L I H M Q T G T P P W V K R Y P R S A Y P S Y L Y I I H K Q A P P L E D I A G D C S P G M R E L I E A A L E R N P N H R P K A A D L L K H E A L N P P R E D Q P R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle.
Alternative names
Cot, Tpl2, Map3k8, Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2
Recommended products
Recombinant mouse MAP3K8/COT protein (His tag) is a Mouse Fragment protein, in the 30 to 397 aa range, expressed in Baculovirus infected Sf9, with >=38% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=38% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 8.5 pmole/min/mg.
Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl2, 0.1 mg/ml BSA) containing 5 μg inactive MEK1, 20 μM ATP, and ab196418 for 60 minutes at 30°C. The amount of ATP transferred was measured.
- Accession
- Q07174-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 8
Preservative: 0.91% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.15% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M E N L Y A S E E P G V Y E P S L M T M Y P D S N Q N E E R S E S L L R S G Q E V P W L S S V R Y G T V E D L L A F A N H V S N M T K H F Y G R R P Q E C G I L L N M V I S P Q N G R Y Q I D S D V L L V P W K L T Y R N I G S G F V P R G A F G K V Y L A Q D M K T K K R M A C K L I P I D Q F K P S D V E I Q A C F R H E N I A E L Y G A V L W G D T V H L F M E A G E G G S V L E K L E S C G P M R E F E I I W V T K H I L K G L D F L H S K K V I H H D I K P S N I V F M S T K A V L V D F G L S V K M T E D V Y L P K D L R G T E I Y M S P E V I L C R G H S T K A D I Y S L G A T L I H M Q T G T P P W V K R Y P R S A Y P S Y L Y I I H K Q A P P L E D I A G D C S P G M R E L I E A A L E R N P N H R P K A A D L L K H E A L N P P R E D Q P R
- Accession
- Q07174
- Protein length
- Fragment
- Predicted molecular weight
- 70 kDa
- Amino acids
- 30 to 397
- Nature
- Recombinant
- Tags
- GST tag N-Terminus, His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle.
- Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
- Post-translational modifications
Autophosphorylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as MAP3K8
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2 product images
SDS-PAGE - Recombinant mouse MAP3K8/COT protein (His tag) (ab196418)
10% SDS-PAGE analysis of 2.5 μg ab196418 with Coomassie staining.
Functional Studies - Recombinant mouse MAP3K8/COT protein (His tag) (ab196418)
Activity assay of ab196418.
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