Recombinant mouse MAP4K1/HPK1 protein (Active) is a Mouse Fragment protein, in the 1 to 346 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A L V D P D I F N K D P R E H Y D L L Q R L G G G T Y G E V F K A R D K V S K D L V A L K M V K M E P D D D V A T L Q K E I L M L K T C R H A N I V A Y H G S Y L W L Q K L W I C M E F C G A G S L Q D I Y Q V T G S L S E L Q I S Y V C R E V L Q G L A Y L H S E K K I H R D I K G A N I L I N D C G E V K L A D F G I S A Q I G A T L A R R L S F I G T P Y W M A P E V A A V A L K G G Y N E L C D I W S L G I T A I E L A E L Q P P L F D V H P L R V L F L M T K S G Y Q P P R L K E K S R W S S S F H N F V K V T L T K N S K K R P S A T K M L S H Q L V S Q P G L N R G L I L D L L D K M K N P G K G L P V D I E D E E P E P P P A I P R R I R S T Y R A S S L G I P D A D C C R R Q M E F Q R P R C V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase, which plays a role in the response to environmental stress (By similarity). Appears to act upstream of the JUN N-terminal pathway (PubMed:9003777). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (By similarity). May play a role in hematopoietic lineage decisions and growth regulation (PubMed:9003777). Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (PubMed:11509653).
Alternative names
Hpk1, Map4k1, Mitogen-activated protein kinase kinase kinase kinase 1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, HPK, MEK kinase kinase 1, MEKKK 1
Recommended products
Recombinant mouse MAP4K1/HPK1 protein (Active) is a Mouse Fragment protein, in the 1 to 346 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268642 was determined to be 70.6 nmol/min/mg in kinase assay using MBP substrate.
- Accession
- P70218-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M A L V D P D I F N K D P R E H Y D L L Q R L G G G T Y G E V F K A R D K V S K D L V A L K M V K M E P D D D V A T L Q K E I L M L K T C R H A N I V A Y H G S Y L W L Q K L W I C M E F C G A G S L Q D I Y Q V T G S L S E L Q I S Y V C R E V L Q G L A Y L H S E K K I H R D I K G A N I L I N D C G E V K L A D F G I S A Q I G A T L A R R L S F I G T P Y W M A P E V A A V A L K G G Y N E L C D I W S L G I T A I E L A E L Q P P L F D V H P L R V L F L M T K S G Y Q P P R L K E K S R W S S S F H N F V K V T L T K N S K K R P S A T K M L S H Q L V S Q P G L N R G L I L D L L D K M K N P G K G L P V D I E D E E P E P P P A I P R R I R S T Y R A S S L G I P D A D C C R R Q M E F Q R P R C V
- Accession
- P70218
- Protein length
- Fragment
- Amino acids
- 1 to 346
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine/threonine-protein kinase, which plays a role in the response to environmental stress (By similarity). Appears to act upstream of the JUN N-terminal pathway (PubMed:9003777). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (By similarity). May play a role in hematopoietic lineage decisions and growth regulation (PubMed:9003777). Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (PubMed:11509653).
- Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
- Post-translational modifications
Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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2 product images
Functional Studies - Recombinant mouse MAP4K1/HPK1 protein (Active) (ab268642)
The specific activity of ab268642 was determined to be 70.6 nmol/min/mg in kinase assay using MBP substrate.
SDS-PAGE - Recombinant mouse MAP4K1/HPK1 protein (Active) (ab268642)
SDS-PAGE analysis of ab268642.
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