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AB268642

Recombinant mouse MAP4K1/HPK1 protein (Active) (GST tag N-Terminus)

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Recombinant mouse MAP4K1/HPK1 protein (Active) (GST tag N-Terminus) is a Mouse Fragment protein, in the 1 to 346 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant mouse MAP4K1/HPK1 protein (Active) (GST tag N-Terminus) (AB268642)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse MAP4K1/HPK1 protein (Active) (GST tag N-Terminus) (AB268642)

The specific activity of ab268642 was determined to be 70.6 nmol/min/mg in kinase assay using MBP substrate.

SDS-PAGE - Recombinant mouse MAP4K1/HPK1 protein (Active) (GST tag N-Terminus) (AB268642)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse MAP4K1/HPK1 protein (Active) (GST tag N-Terminus) (AB268642)

SDS-PAGE analysis of ab268642.

Key facts

Purity

>90% SDS-PAGE

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab268642 was determined to be 70.6 nmol/min/mg in kinase assay using MBP substrate.

Accession

P70218-1

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MALVDPDIFNKDPREHYDLLQRLGGGTYGEVFKARDKVSKDLVALKMVKMEPDDDVATLQKEILMLKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSEKKIHRDIKGANILINDCGEVKLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKSRWSSSFHNFVKVTLTKNSKKRPSATKMLSHQLVSQPGLNRGLILDLLDKMKNPGKGLPVDIEDEEPEPPPAIPRRIRSTYRASSLGIPDADCCRRQMEFQRPRCV","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":346,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P70218","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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General info

Function

Serine/threonine-protein kinase, which plays a role in the response to environmental stress (By similarity). Appears to act upstream of the JUN N-terminal pathway (PubMed : 9003777). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (By similarity). May play a role in hematopoietic lineage decisions and growth regulation (PubMed : 9003777). Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (PubMed : 11509653).

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Post-translational modifications

Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.. Tyrosine-phosphorylated after activation of hemopoietic cells.. Ubiquitinated by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex following autophosphorylation, leading to its degradation by the proteasome.

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Product protocols

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Target data

Serine/threonine-protein kinase, which plays a role in the response to environmental stress (By similarity). Appears to act upstream of the JUN N-terminal pathway (PubMed : 9003777). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (By similarity). May play a role in hematopoietic lineage decisions and growth regulation (PubMed : 9003777). Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (PubMed : 11509653).
See full target information Map4k1

Alternative Names

Hpk1, Map4k1, Mitogen-activated protein kinase kinase kinase kinase 1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, HPK, MEK kinase kinase 1, MEKKK 1
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