Recombinant mouse MDH2 protein (Active) (His tag N-Terminus)
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Recombinant mouse MDH2 protein (Active) (His tag N-Terminus) is a Mouse Full Length protein, in the 25 to 338 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, Mass Spec.
View Alternative Names
Mor1, Mdh2
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant mouse MDH2 protein (Active) (His tag N-Terminus) (AB229176)
15% SDS-PAGE analysis of 3 μg ab229176.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MDH2 participates in the critical process of energy production within the cell. While it does not form a complex itself its activity is intimately connected with other enzymes in mitochondrial energy metabolism. The malate dehydrogenase assay often measures the activity of MDH2 to understand the metabolic status of cells. By facilitating the oxidation of malate MDH2 aids in maintaining the efficiency of the mitochondrial electron transport chain by regenerating NADH.
Pathways
The enzyme is essential in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle MDH2 collaborates with enzymes like citrate synthase and isocitrate dehydrogenase to assist in the conversion of acetyl-CoA into energy-rich molecules. The malate-aspartate shuttle on the other hand involves MDH2 working closely with aspartate transaminase to transfer reducing equivalents into the mitochondria. These pathways highlight MDH2’s importance in cellular energy homeostasis.
Specifications
Form
Liquid
Additional notes
ab229176 was purified using conventional chromatography techniques.
General info
Sequence similarities
Belongs to the LDH/MDH superfamily. MDH type 1 family.
Post-translational modifications
Acetylation is enhanced after treatment either with trichostin A (TCA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation (By similarity). Acetylation of Lys-239 and Lys-314 is observed in liver mitochondria from fasted mice but not from fed mice.
Subcellular localisation
Mitochondrion matrix
Target data
Complementary Products
Product promise
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