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AB317873

Recombinant Mouse MIF Protein (His-tag)

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Recombinant Mouse MIF Protein (His-tag) is a Mouse Full Length protein, expressed in HEK 293 cells, with >95%, <1.39 EU/mg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE.

View Alternative Names

Macrophage migration inhibitory factor, MIF, Delayed early response protein 6, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, DER6, GIF, Mif

3 Images
Mass Spectrometry - Recombinant Mouse MIF Protein (His-tag) (AB317873)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Mouse MIF Protein (His-tag) (AB317873)

Mass determination by ESI-TOF. Predicted MW is 14550 Da (+/- 10 Da by ESI-TOF). Observed MW is 14552.36 Da.

SDS-PAGE - Recombinant Mouse MIF Protein (His-tag) (AB317873)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse MIF Protein (His-tag) (AB317873)

SDS-PAGE analysis of ab317873 under reducing conditions for 2ug protein.

HPLC - Recombinant Mouse MIF Protein (His-tag) (AB317873)
  • HPLC

Supplier Data

HPLC - Recombinant Mouse MIF Protein (His-tag) (AB317873)

HPLC analysis of ab317873

Key facts

Purity

>95% HPLC

Endotoxin level

<1.39 EU/mg

Expression system

HEK 293 cells

Tags

His tag N-Terminus

Applications

Mass Spec, HPLC, SDS-PAGE

applications

Biologically active

No

Mass Spectrometry

LC-MS/MS

Accession

P34884

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"PMFIVNTNVPRASVPEGFLSELTQQLAQATGKPAQYIAVHVVPDQLMTFSGTNDPCALCSLHSIGKIGGAQNRNYSKLLCGLLSDRLHISPDRVYINYYDMNAANVGWNGSTFA","proteinLength":"Full Length","predictedMolecularWeight":"14.55 kDa","actualMolecularWeight":"14.55 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P34884","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.
Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.
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Specifications

Form

Lyophilized

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General info

Function

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (By similarity). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (By similarity). Counteracts the anti-inflammatory activity of glucocorticoids (By similarity). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed : 10933783, PubMed : 16780921, PubMed : 19188446). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed : 10933783, PubMed : 16780921, PubMed : 19188446).

Sequence similarities

Belongs to the MIF family.

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Product protocols

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Target data

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (By similarity). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (By similarity). Counteracts the anti-inflammatory activity of glucocorticoids (By similarity). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed : 10933783, PubMed : 16780921, PubMed : 19188446). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed : 10933783, PubMed : 16780921, PubMed : 19188446).
See full target information Mif
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