Recombinant mouse MSK2 / RSK-B protein
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant mouse MSK2 / RSK-B protein is a Mouse Full Length protein, in the 1 to 773 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB, FuncS.
View Alternative Names
Msk2, Rps6ka4, Ribosomal protein S6 kinase alpha-4, S6K-alpha-4, 90 kDa ribosomal protein S6 kinase 4, Nuclear mitogen- and stress-activated protein kinase 2, RSK-like protein kinase, RLSK
- FuncS
Unknown
Functional Studies - Recombinant mouse MSK2 / RSK-B protein (AB125610)
The specific activity of ab125610 was determined to be 9 nmol/mg/min by Kinase Assay.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant mouse MSK2 / RSK-B protein (AB125610)
SDS-PAGE analysis of ab125610.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This kinase involves itself in the regulation of transcription by phosphorylating CREB and activating histone acetylation. MSK2 acts in concert with other kinases and proteins forming part of larger signaling complexes that modulate gene expression and chromatin states. It also impacts inflammation by activating specific pathways that target inflammatory gene expression serving as a connection point between various cellular signals and responses.
Pathways
MSK2 plays a significant role in the MAPK signaling pathway and the NF-kB pathway. These pathways are pivotal in cell differentiation proliferation and stress responses. MSK2 often functions in conjunction with proteins like ERK1/2 and p38 MAPK facilitating the downstream effects of these signaling molecules. In particular MSK2's phosphorylation activities have direct effects on transcription factors within these pathways impacting cellular fate decisions.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity).
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Post-translational modifications
Ser-343 and Thr-568 phosphorylation is required for kinase activity. Ser-343 and Ser-196 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-343, Thr-568 and Thr-687 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-737 and Ser-745 by the N-terminal kinase domain (By similarity).
Subcellular localisation
Nucleus
Target data
Complementary Products
![Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] (AB81289)](https://content.abcam.com/products/images/cd34-antibody-ep373y-ab81289--flow-cytometry-intracellular-img439652.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com