Recombinant Mouse MUSK protein (Tagged) is a Mouse Fragment protein, in the 22 to 468 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
E K L P K A P V I T T P L E T V D A L V E E V A T F M C A V E S Y P Q P E I S W T R N K I L I K L F D T R Y S I R E N G Q L L T I L S V E D S D D G I Y C C I A N N G V G G A V E S C G A L Q V K M K P K I T R P P I N V K I I E G L K A V L P C T T M G N P K P S V S W I K G D N A L R E N S R I A V L E S G S L R I H N V Q K E D A G Q Y R C V A K N S L G T A Y S K L V K L E V E V F A R I L R A P E S H N V T F G S F V T L R C T A I G I P V P T I S W I E N G N A V S S G S I Q E S V K D R V I D S R L Q L F I T K P G L Y T C I A T N K H G E K F S T A K A A A T V S I A E W S K S Q K D S Q G Y C A Q Y R G E V C D A V L A K D A L V F F N T S Y R D P E D A Q E L L I H T A W N E L K A V S P L C R P A A E A L L C N H L F Q E C S P G V V P T P M P I C R E Y C L A V K E L F C A K E W Q A M E G K A H R G L Y R S G M H L L P V P E C S K L P S M H R D P T A C T R L P Y L D Y K K E N I T T F P S I T S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.
Alternative names
Nsk2, Musk, Muscle-specific tyrosine-protein kinase receptor, MuSK, Muscle-specific kinase receptor
Recommended products
Recombinant Mouse MUSK protein (Tagged) is a Mouse Fragment protein, in the 22 to 468 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q61006-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- E K L P K A P V I T T P L E T V D A L V E E V A T F M C A V E S Y P Q P E I S W T R N K I L I K L F D T R Y S I R E N G Q L L T I L S V E D S D D G I Y C C I A N N G V G G A V E S C G A L Q V K M K P K I T R P P I N V K I I E G L K A V L P C T T M G N P K P S V S W I K G D N A L R E N S R I A V L E S G S L R I H N V Q K E D A G Q Y R C V A K N S L G T A Y S K L V K L E V E V F A R I L R A P E S H N V T F G S F V T L R C T A I G I P V P T I S W I E N G N A V S S G S I Q E S V K D R V I D S R L Q L F I T K P G L Y T C I A T N K H G E K F S T A K A A A T V S I A E W S K S Q K D S Q G Y C A Q Y R G E V C D A V L A K D A L V F F N T S Y R D P E D A Q E L L I H T A W N E L K A V S P L C R P A A E A L L C N H L F Q E C S P G V V P T P M P I C R E Y C L A V K E L F C A K E W Q A M E G K A H R G L Y R S G M H L L P V P E C S K L P S M H R D P T A C T R L P Y L D Y K K E N I T T F P S I T S
- Accession
- Q61006
- Protein length
- Fragment
- Predicted molecular weight
- 69.3 kDa
- Amino acids
- 22 to 468
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
- Post-translational modifications
Ubiquitinated by PDZRN3. Ubiquitination promotes endocytosis and lysosomal degradation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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3 product images
SDS-PAGE - Recombinant Mouse MUSK protein (Tagged) (ab226426)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226426 with 5% enrichment gel and 15% separation gel.
Mass Spectrometry - Recombinant Mouse MUSK protein (Tagged) (ab226426)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab226426 could indicate that this peptide derived from E.coli-expressed mouse Musk.
Mass Spectrometry - Recombinant Mouse MUSK protein (Tagged) (ab226426)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab226426 could indicate that this peptide derived from E.coli-expressed mouse Musk.
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