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Recombinant Mouse NEK1 protein is a Mouse Fragment protein, in the 1 to 495 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB, FuncS.
Alternative names=Serine/threonine-protein kinase Nek1, Never in mitosis A-related kinase 1, NimA-related protein kinase 1, Nek1
- Purity
>70% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Mouse NEK1 protein is a Mouse Fragment protein, in the 1 to 495 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB, FuncS.
Alternative names=Serine/threonine-protein kinase Nek1, Never in mitosis A-related kinase 1, NimA-related protein kinase 1, Nek1
Key facts
- Purity
>70% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
- Biological activity
The specific activity of ab133146 was determined to be 70 nmol/min/mg.
- Predicted molecular weight
56.62 kDa
- Amino acids
1 to 495
- Accession
P51954
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Mouse
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.31% Glutathione, 0.005% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Specifications
- Form
Liquid
General info
- Function
Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity (PubMed:1382974). Involved in DNA damage checkpoint control and for proper DNA damage repair (PubMed:18843199). In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (By similarity). May be implicated in the control of meiosis (PubMed:1382974). Involved in cilium assembly (By similarity).
- Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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