Recombinant Mouse Neuroligin 1 protein (His tag) is a Mouse Fragment protein, in the 1 to 697 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A L P R C M W P N Y V W R A M M A C V V H R G S G A P L T L C L L G C L L Q T F H V L S Q K L D D V D P L V T T N F G K I R G I K K E L N N E I L G P V I Q F L G V P Y A A P P T G E H R F Q P P E P P S P W S D I R N A T Q F A P V C P Q N I I D G R L P E V M L P V W F T N N L D V V S S Y V Q D Q S E D C L Y L N I Y V P T E D G P L T K K H T D D L G D N D G A E D E D I R D S G G P K P V M V Y I H G G S Y M E G T G N L Y D G S V L A S Y G N V I V I T V N Y R L G V L G F L S T G D Q A A K G N Y G L L D L I Q A L R W T S E N I G F F G G D P L R I T V F G S G A G G S C V N L L T L S H Y S E G N R W S N S T K G L F Q R A I A Q S G T A L S S W A V S F Q P A K Y A R I L A T K V G C N V S D T V E L V E C L Q K K P Y K E L V D Q D V Q P A R Y H I A F G P V I D G D V I P D D P Q I L M E Q G E F L N Y D I M L G V N Q G E G L K F V E N I V D S D D G V S A S D F D F A V S N F V D N L Y G Y P E G K D V L R E T I K F M Y T D W A D R H N P E T R R K T L L A L F T D H Q W V A P A V A T A D L H S N F G S P T Y F Y A F Y H H C Q T D Q V P A W A D A A H G D E V P Y V L G I P M I G P T E L F P C N F S K N D V M L S A V V M T Y W T N F A K T G D P N Q P V P Q D T K F I H T K P N R F E E V A W T R Y S Q K D Q L Y L H I G L K P R V K E H Y R A N K V N L W L E L V P H L H N L N D I S Q Y T S T T T K V P S T D I T L R P T R K N S T P V T S A F P T A K Q D D P K Q Q P S P F S V D Q R D Y S T E L S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep (PubMed:23716671). The protein is involved in nervous system development.
Alternative names
Kiaa1070, Nlgn1, Neuroligin-1
Recommended products
Recombinant Mouse Neuroligin 1 protein (His tag) is a Mouse Fragment protein, in the 1 to 697 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q99K10-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M A L P R C M W P N Y V W R A M M A C V V H R G S G A P L T L C L L G C L L Q T F H V L S Q K L D D V D P L V T T N F G K I R G I K K E L N N E I L G P V I Q F L G V P Y A A P P T G E H R F Q P P E P P S P W S D I R N A T Q F A P V C P Q N I I D G R L P E V M L P V W F T N N L D V V S S Y V Q D Q S E D C L Y L N I Y V P T E D G P L T K K H T D D L G D N D G A E D E D I R D S G G P K P V M V Y I H G G S Y M E G T G N L Y D G S V L A S Y G N V I V I T V N Y R L G V L G F L S T G D Q A A K G N Y G L L D L I Q A L R W T S E N I G F F G G D P L R I T V F G S G A G G S C V N L L T L S H Y S E G N R W S N S T K G L F Q R A I A Q S G T A L S S W A V S F Q P A K Y A R I L A T K V G C N V S D T V E L V E C L Q K K P Y K E L V D Q D V Q P A R Y H I A F G P V I D G D V I P D D P Q I L M E Q G E F L N Y D I M L G V N Q G E G L K F V E N I V D S D D G V S A S D F D F A V S N F V D N L Y G Y P E G K D V L R E T I K F M Y T D W A D R H N P E T R R K T L L A L F T D H Q W V A P A V A T A D L H S N F G S P T Y F Y A F Y H H C Q T D Q V P A W A D A A H G D E V P Y V L G I P M I G P T E L F P C N F S K N D V M L S A V V M T Y W T N F A K T G D P N Q P V P Q D T K F I H T K P N R F E E V A W T R Y S Q K D Q L Y L H I G L K P R V K E H Y R A N K V N L W L E L V P H L H N L N D I S Q Y T S T T T K V P S T D I T L R P T R K N S T P V T S A F P T A K Q D D P K Q Q P S P F S V D Q R D Y S T E L S
- Accession
- Q99K10
- Protein length
- Fragment
- Predicted molecular weight
- 73.5 kDa
- Amino acids
- 1 to 697
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep (PubMed:23716671). The protein is involved in nervous system development.
- Sequence similarities
Belongs to the type-B carboxylesterase/lipase family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neuroligin 1 also known as NLGN1 is a member of the neuroligin family of cell adhesion molecules. It plays a significant mechanical role at synaptic junctions where it mediates synapse formation and maintenance. Neuroligin 1 weighs approximately 97 kDa and is mainly expressed in the brain particularly in postsynaptic densities of excitatory glutamatergic synapses. Neuroligin 1 interacts with neurexins on the presynaptic side facilitating trans-synaptic connections important for proper neuronal communication.
- Biological function summary
Neuroligin 1 contributes to synaptic organization and signaling by being part of a larger protein complex. This complex involves postsynaptic scaffolding proteins such as PSD-95 which anchor neuroligin to the cytoskeleton and receptors like NMDA and AMPA receptors. Neuroligin 1 regulates synaptic stabilization and plasticity which are essential for learning and memory processes. Through these interactions it impacts synaptic strength and efficiency playing an integral role in the modulation of synaptic activity.
- Pathways
Neuroligin 1 is a critical component of synaptic development and function pathways. It operates within the synaptic signaling and plasticity pathways affecting neurotransmitter function and reception. Neuroligin 1 functions in coordination with proteins such as neurexins and SHANK proteins to regulate the assembly and maturation of synapses. These interactions influence calcium signaling and involve the recruitment of other synaptic proteins that further contribute to synaptic modulation.
- Associated diseases and disorders
Neuroligin 1 is linked to neurological conditions like autism spectrum disorders (ASD) and schizophrenia. Mutations or dysregulations of neuroligin 1 impact synaptic connectivity leading to altered neuronal networks and communication deficits involved in these conditions. The balance of excitatory and inhibitory synaptic function that neuroligin and its binding partners including neurexins and SHANK3 control is directly implicated in the pathophysiology of these disorders. Disruptions in neuroligin-neurexin signaling pathways may therefore contribute to the synaptic dysfunctions observed in ASD and schizophrenia.
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SDS-PAGE - Recombinant Mouse Neuroligin 1 protein (His tag) (ab276916)
SDS-PAGE analysis of ab276916
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