Recombinant mouse NSE protein (Active) is a Mouse Full Length protein, in the 1 to 434 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS, MS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S I E K I W A R E I L D S R G N P T V E V D L Y T A K G L F R A A V P S G A S T G I Y E A L E L R D G D K Q R Y L G K G V L K A V D H I N S R I A P A L I S S G I S V V E Q E K L D N L M L E L D G T E N K S K F G A N A I L G V S L A V C K A G A A E R D L P L Y R H I A Q L A G N S D L I L P V P A F N V I N G G S H A G N K L A M Q E F M I L P V G A E S F R D A M R L G A E V Y H T L K G V I K D K Y G K D A T N V G D E G G F A P N I L E N S E A L E L V K E A I D K A G Y T E K M V I G M D V A A S E F Y R D G K Y D L D F K S P A D P S R Y I T G D Q L G A L Y Q D F V R N Y P V V S I E D P F D Q D D W A A W S K F T A N V G I Q I V G D D L T V T N P K R I E R A V E E K A C N C L L L K V N Q I G S V T E A I Q A C K L A Q E N G W G V M V S H R S G E T E D T F I A D L V V G L C T G Q I K T G A P C R S E R L A K Y N Q L M R I E E E L G D E A R F A G H N F R N P S V L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Alternative names
Eno-2, Eno2, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE
Recommended products
Recombinant mouse NSE protein (Active) is a Mouse Full Length protein, in the 1 to 434 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS, MS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 10,000 pmol/min/μg, and was obtained by measuring the decrease of NAD in absorbance at 340 nm resulting from NADH at pH 6.5 at 37°C.
- Accession
- P17183-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S I E K I W A R E I L D S R G N P T V E V D L Y T A K G L F R A A V P S G A S T G I Y E A L E L R D G D K Q R Y L G K G V L K A V D H I N S R I A P A L I S S G I S V V E Q E K L D N L M L E L D G T E N K S K F G A N A I L G V S L A V C K A G A A E R D L P L Y R H I A Q L A G N S D L I L P V P A F N V I N G G S H A G N K L A M Q E F M I L P V G A E S F R D A M R L G A E V Y H T L K G V I K D K Y G K D A T N V G D E G G F A P N I L E N S E A L E L V K E A I D K A G Y T E K M V I G M D V A A S E F Y R D G K Y D L D F K S P A D P S R Y I T G D Q L G A L Y Q D F V R N Y P V V S I E D P F D Q D D W A A W S K F T A N V G I Q I V G D D L T V T N P K R I E R A V E E K A C N C L L L K V N Q I G S V T E A I Q A C K L A Q E N G W G V M V S H R S G E T E D T F I A D L V V G L C T G Q I K T G A P C R S E R L A K Y N Q L M R I E E E L G D E A R F A G H N F R N P S V L
- Accession
- P17183
- Protein length
- Full Length
- Predicted molecular weight
- 49.7 kDa
- Amino acids
- 1 to 434
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab222947 was purified using conventional chromatography techniques.
General info
- Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
- Sequence similarities
Belongs to the enolase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.
- Biological function summary
NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.
- Pathways
NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.
- Associated diseases and disorders
NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.
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1 product image
SDS-PAGE - Recombinant mouse NSE protein (Active) (ab222947)
15% SDS-PAGE analysis of 3 μg ab222947.
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