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AB78707

Recombinant Mouse NUCB2 protein (His tag N-Terminus)

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Recombinant Mouse NUCB2 protein (His tag N-Terminus) is a Mouse Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

Nefa, Nucleobindin-2, DNA-binding protein NEFA, Prepronesfatin

1 Images
SDS-PAGE - Recombinant Mouse NUCB2 protein (AB78707)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Mouse NUCB2 protein (AB78707)

15% SDS-PAGE showing ab78707 at approximately 49kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P81117

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 8 Constituents: 0.58% Sodium chloride, 0.242% Tris, 0.0292% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as Nucleobindin 2.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMVPIDVDKTKVHNTEPVENARIEPPDTGLYYDEYLKQVIEVLETDPHFREKLQKADIEEIRSGRLSQELDLVSHKVRTRLDELKRQEVGRLRMLIKAKLDALQDTGMNHHLLLKQFEHLNHQNPNTFESRDLDMLIKAATADLEQYDRTRHEEFKKYEMMKEHERREYLKTLSEEKRKEEESKFEEMKRKHEDHPKVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNNDGFLDEQELEALFTRELEKVYNPQNAEDDMIEMEEERLRMREHVMSEIDNNKDRLVTLEEFLRATEKKEFLEPDSWETLDQQQLFTEDELKEYESIIAIQENELKKRAEELQKQKEDLQRQHDHLEAQKQEYHQAVQHLEQKKLQQGIAPSGPAGELKFEPHT","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P81117","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUCB2 also known as nesfatin-1 precursor is a calcium-binding protein involved in various physiological processes. This 50-kDa protein integrates signals related to energy balance and is important for appetite regulation. NUCB2 is expressed in several tissues such as the hypothalamus pancreas and adipose tissue which suggests its involvement in widespread bodily functions. The protein is produced as an 82-kDa precursor protein cleaved to release active fragments like nesfatin-1 which possesses biological activity.
Biological function summary

This protein influences food intake and energy homeostasis. NUCB2 is part of a signaling complex that modulates cellular responses to metabolic needs. It functions as a satiety molecule signaling when the body does not require additional food intake. The protein's regulatory role in appetite makes it important for maintaining body weight and energy utilization. NUCB2’s expression in specific brain areas particularly indicates its role in central nervous system pathways that control energy use.

Pathways

NUCB2 engages in vital neural circuits governing appetite and energy expenditure. It connects with the melanocortin and neuropeptide Y pathways which are central to maintaining energy balance and body weight. The interaction of NUCB2 with neuropeptide Y an essential regulator of hunger exemplifies how it modulates these pathways despite its main role not being in leptin signaling.

Disturbances in NUCB2 are linked to obesity and stress-related conditions. Obesity involves disrupted signaling where NUCB2’s role as a satiety signal becomes critical. The protein also plays a part in stress-related pathways influencing stress-induced appetite changes which can relate to conditions like depression. Alterations in NUCB2 expression or function can lead to abnormal body weight and stress responses making it a potential target for therapeutic interventions in these conditions.
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Specifications

Form

Liquid

Additional notes

ab78707 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

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General info

Function

Calcium-binding protein which may have a role in calcium homeostasis (PubMed : 10915798). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (By similarity).. Nesfatin-1. Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.

Sequence similarities

Belongs to the nucleobindin family.

Subcellular localisation

Nucleus envelope

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Product protocols

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Target data

Calcium-binding protein which may have a role in calcium homeostasis (PubMed : 10915798). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (By similarity).. Nesfatin-1. Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.
See full target information Nucb2
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