Recombinant Mouse Ornithine Carbamoyltransferase/OTC protein (Tagged) is a Mouse Full Length protein, in the 33 to 354 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S Q V Q L K G R D L L T L K N F T G E E I Q Y M L W L S A D L K F R I K Q K G E Y L P L L Q G K S L G M I F E K R S T R T R L S T E T G F A L L G G H P S F L T T Q D I H L G V N E S L T D T A R V L S S M T D A V L A R V Y K Q S D L D T L A K E A S I P I V N G L S D L Y H P I Q I L A D Y L T L Q E H Y G S L K G L T L S W I G D G N N I L H S I M M S A A K F G M H L Q A A T P K G Y E P D P N I V K L A E Q Y A K E N G T K L S M T N D P L E A A R G G N V L I T D T W I S M G Q E D E K K K R L Q A F Q G Y Q V T M K T A K V A A S D W T F L H C L P R K P E E V D D E V F Y S P R S L V F P E A E N R K W T I M A V M V S L L T D Y S P V L Q K P K F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline. The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion.
Alternative names
OTCase, Otc
Recommended products
Recombinant Mouse Ornithine Carbamoyltransferase/OTC protein (Tagged) is a Mouse Full Length protein, in the 33 to 354 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P11725-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- S Q V Q L K G R D L L T L K N F T G E E I Q Y M L W L S A D L K F R I K Q K G E Y L P L L Q G K S L G M I F E K R S T R T R L S T E T G F A L L G G H P S F L T T Q D I H L G V N E S L T D T A R V L S S M T D A V L A R V Y K Q S D L D T L A K E A S I P I V N G L S D L Y H P I Q I L A D Y L T L Q E H Y G S L K G L T L S W I G D G N N I L H S I M M S A A K F G M H L Q A A T P K G Y E P D P N I V K L A E Q Y A K E N G T K L S M T N D P L E A A R G G N V L I T D T W I S M G Q E D E K K K R L Q A F Q G Y Q V T M K T A K V A A S D W T F L H C L P R K P E E V D D E V F Y S P R S L V F P E A E N R K W T I M A V M V S L L T D Y S P V L Q K P K F
- Accession
- P11725
- Protein length
- Full Length
- Predicted molecular weight
- 52.1 kDa
- Amino acids
- 33 to 354
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline. The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion.
- Sequence similarities
Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.
- Post-translational modifications
Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as Ornithine Carbamoyltransferase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ornithine Carbamoyltransferase (OTC) also known as ornithine aminotransferase is an enzyme critical in the urea cycle. The OTC protein facilitates the conversion of ornithine and carbamoyl phosphate into citrulline a process essential for nitrogen excretion. The human OTC protein has a mass of approximately 36 kilodaltons and is primarily expressed in the liver mitochondria. Protein assays and procurement efforts often focus on obtaining accurate measurements and ensuring high-purity OTC peptides enabling proper functional studies.
- Biological function summary
Ornithine carbamoyltransferase plays a significant role in detoxifying ammonia by forming citrulline an important urea cycle intermediate. The protein functions as a homotrimer meaning three identical subunits form an active enzyme complex. This multimeric state facilitates efficient substrate processing allowing effective handling of nitrogen waste. The integral role of OTC in the urea cycle highlights its importance in maintaining systemic nitrogen balance.
- Pathways
OTC is a central component of the urea cycle and influences nitrogen metabolism pathways. These pathways include the conversion of ammonia to urea an important detoxifying process for safe excretion by the kidneys. OTC closely interacts with enzymes such as argininosuccinate synthetase highlighting the coordinated action required to advance urea formation. Understanding OTC's participation within these pathways helps identify its regulatory points and the potential impact of genetic variations.
- Associated diseases and disorders
Mutations in the OTC gene can lead to ornithine transcarbamylase deficiency a disorder affecting ammonia detoxification. This X-linked genetic condition disrupts normal nitrogen metabolism causing ammonia accumulation particularly harmful to the central nervous system. OTC deficiency shares a functional relationship with other urea cycle disorders such as citrullinemia due to its contribution to the same biochemical pathway. Researchers focus on identifying OTC protein mutations to improve diagnosis and treatment of these conditions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Mouse Ornithine Carbamoyltransferase/OTC protein (Tagged) (ab235795)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab235795.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com