Recombinant Mouse OSTM1 protein (His tag) is a Mouse Fragment protein, in the 1 to 288 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A R D A E L A R S S G W P W R W L P A L L L L Q L L R W R C A L C A L P F T S S R H P G F A D L L S E Q Q L L E V Q D L T L S L L Q G G G L G P L S L L P P D L P D L E P E C R E L L M D F A N S S A E L T A C M V R S A R P V R L C Q T C Y P L F Q Q V A I K M D N I S R N I G N T S E G P R C G G S L L T A D R M Q I V L M V S E F F N S T W Q E A N C A N C L T N N G E D L S N N T E D F L S L F N K T L A C F E H N L Q G H T Y S L L P P K N Y S E V C R N C K E A Y K N L S L L Y S Q M Q K L N G L E N K A E P E T H L C I D V E D A M N I T R K L W S R T F N C S V T C S D T V S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Required for osteoclast and melanocyte maturation and function.
Alternative names
Gl, Ostm1, Osteopetrosis-associated transmembrane protein 1, Chloride channel 7 beta subunit, Grey-lethal protein
Recommended products
Recombinant Mouse OSTM1 protein (His tag) is a Mouse Fragment protein, in the 1 to 288 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8BGT0-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M A R D A E L A R S S G W P W R W L P A L L L L Q L L R W R C A L C A L P F T S S R H P G F A D L L S E Q Q L L E V Q D L T L S L L Q G G G L G P L S L L P P D L P D L E P E C R E L L M D F A N S S A E L T A C M V R S A R P V R L C Q T C Y P L F Q Q V A I K M D N I S R N I G N T S E G P R C G G S L L T A D R M Q I V L M V S E F F N S T W Q E A N C A N C L T N N G E D L S N N T E D F L S L F N K T L A C F E H N L Q G H T Y S L L P P K N Y S E V C R N C K E A Y K N L S L L Y S Q M Q K L N G L E N K A E P E T H L C I D V E D A M N I T R K L W S R T F N C S V T C S D T V S
- Accession
- Q8BGT0
- Protein length
- Fragment
- Predicted molecular weight
- 29.7 kDa
- Amino acids
- 1 to 288
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Required for osteoclast and melanocyte maturation and function.
- Sequence similarities
Belongs to the OSTM1 family.
- Post-translational modifications
Undergoes proteolytic cleavage in the luminal domain, the cleaved fragments might be linked by disulfide bonds with the remnant of the protein.
- Subcellular localisation
- Lysosome membrane
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OSTM1 also known as Osteopetrosis Associated Transmembrane Protein 1 is a protein with a molecular mass of approximately 47 kDa. It primarily resides in lysosomal and endosomal membranes. OSTM1 is highly expressed in osteoclasts cells responsible for bone resorption. It is important for osteoclast differentiation and function ensuring the breakdown of the bone matrix. OSTM1 forms a complex with other membrane proteins assisting in ion homeostasis within the osteoclast environment.
- Biological function summary
The OSTM1 protein contributes to maintaining cellular ionic balance and is a part of the chloride channel complex. This complex is important for proper osteoclast activity as it facilitates the transport of ions necessary for creating an acidic environment. The protein participates in chloride-ion channel transport affecting the acidification of resorptive vacuoles. This action supports bone resorption enabling bone remodeling and growth.
- Pathways
OSTM1 plays an important role in the RANK-RANKL signaling pathway which regulates osteoclast differentiation and activation. OSTM1 interacts with proteins like CLCN7 another chloride channel protein within this pathway. Through this interaction it assists in the formation and maintenance of acidic compartments in osteoclasts essential for bone mineral dissolution. Therefore OSTM1 is integral to the processes of bone turnover and mineral homeostasis.
- Associated diseases and disorders
Mutations or malfunctions in OSTM1 are directly linked to the development of autosomal recessive osteopetrosis a condition characterized by increased bone density and associated complications. OSTM1 dysfunction leads to impaired osteoclast activity resulting in defective bone resorption. Additionally a connection exists between OSTM1 and CLCN7 where defects in either protein can manifest similar clinical conditions highlighting their collaborative role in maintaining skeletal health.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Mouse OSTM1 protein (His tag) (ab277009)
SDS-PAGE analysis of ab277009
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com