Recombinant mouse PD1 protein (Active) is a Mouse Fragment protein, in the 25 to 167 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
>90% SDS-PAGE
< 1 EU/µg
HEK 293 cells
His tag C-Terminus
SDS-PAGE, FuncS
Yes
L E V P N G P W R S L T F Y P A W L T V S E G A N A T F T C S L S N W S E D L M L N W N R L S P S N Q T E K Q A A F C N G L S Q P V Q D A R F Q I I Q L P N R H D F H M N I L D T R R N D S G I Y L C G A I S L H P K A K I E E S P G A E L V V T E R I L E T S T R Y P S P S P K P E G R F Q
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Protein migrates as 25 - 45 kDa due to different glycosylation. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11209085, PubMed:11698646, PubMed:21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:18287011, PubMed:18641123, PubMed:22641383). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).
Pd1, Pd1, Pdcd1, Programmed cell death protein 1, Protein PD-1, mPD-1
Recombinant mouse PD1 protein (Active) is a Mouse Fragment protein, in the 25 to 167 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
>90% SDS-PAGE
< 1 EU/µg
HEK 293 cells
His tag C-Terminus
SDS-PAGE, FuncS
Yes
Loaded mouse PD-L1, Fc Tag on Protein A Biosensor, can bind ab180051 with an affinity constant of 6.9 μM as determined in BLI assay.
No
Mouse
Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
L E V P N G P W R S L T F Y P A W L T V S E G A N A T F T C S L S N W S E D L M L N W N R L S P S N Q T E K Q A A F C N G L S Q P V Q D A R F Q I I Q L P N R H D F H M N I L D T R R N D S G I Y L C G A I S L H P K A K I E E S P G A E L V V T E R I L E T S T R Y P S P S P K P E G R F Q
Fragment
16.9 kDa
25 to 167
Recombinant
His tag C-Terminus
Lyophilized
Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11209085, PubMed:11698646, PubMed:21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:18287011, PubMed:18641123, PubMed:22641383). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).
Ubiquitinated at Lys-235 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains.
Blue Ice
Up to 12 months
+4°C
-20°C
Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Endotoxin Levels determined by LAL method.
This supplementary information is collated from multiple sources and compiled automatically.
PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.
PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.
PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.
PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.
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Terms & Conditions.
Reduced ab180051 on SDS-PAGE, stained overnight with Coomassie Blue.
The protein migrates as 25-45 kDa under reducing condition due to glycosylation.
Purity of protein >90%.
Loaded Mouse PD-L1, Fc Tag on Protein A Biosensor, can bind Mouse PD-1, His Tag (ab180051) with an affinity constant of 6.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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