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Recombinant mouse PD1 protein (Active) is a Mouse Fragment protein, in the 25 to 167 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

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Images

SDS-PAGE - Recombinant mouse PD1 protein (Active) (AB180051), expandable thumbnail
  • Biological Activity - Recombinant mouse PD1 protein (Active) (AB180051), expandable thumbnail

Publications

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

L E V P N G P W R S L T F Y P A W L T V S E G A N A T F T C S L S N W S E D L M L N W N R L S P S N Q T E K Q A A F C N G L S Q P V Q D A R F Q I I Q L P N R H D F H M N I L D T R R N D S G I Y L C G A I S L H P K A K I E E S P G A E L V V T E R I L E T S T R Y P S P S P K P E G R F Q

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

Protein migrates as 25 - 45 kDa due to different glycosylation.

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11209085, PubMed:11698646, PubMed:21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:18287011, PubMed:18641123, PubMed:22641383). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).

Alternative names

Recommended products

Recombinant mouse PD1 protein (Active) is a Mouse Fragment protein, in the 25 to 167 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS

Biological activity

Loaded mouse PD-L1, Fc Tag on Protein A Biosensor, can bind ab180051 with an affinity constant of 6.9 μM as determined in BLI assay.

Accession
Q02242-1
Animal free

No

Species

Mouse

Reconstitution

Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 95% PBS, 5% Trehalose

Sequence info

Amino acid sequence

L E V P N G P W R S L T F Y P A W L T V S E G A N A T F T C S L S N W S E D L M L N W N R L S P S N Q T E K Q A A F C N G L S Q P V Q D A R F Q I I Q L P N R H D F H M N I L D T R R N D S G I Y L C G A I S L H P K A K I E E S P G A E L V V T E R I L E T S T R Y P S P S P K P E G R F Q

Accession

Q02242

Protein length

Fragment

Predicted molecular weight

16.9 kDa

Amino acids

25 to 167

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Lyophilized

General info

Function

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11209085, PubMed:11698646, PubMed:21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:18287011, PubMed:18641123, PubMed:22641383). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).

Post-translational modifications

Ubiquitinated at Lys-235 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Endotoxin Levels determined by LAL method.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.

Biological function summary

PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.

Pathways

PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.

Associated diseases and disorders

PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.

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2 product images

  • SDS-PAGE - Recombinant mouse PD1 protein (Active) (ab180051), expandable thumbnail

    SDS-PAGE - Recombinant mouse PD1 protein (Active) (ab180051)

    Reduced ab180051 on SDS-PAGE, stained overnight with Coomassie Blue.

    The protein migrates as 25-45 kDa under reducing condition due to glycosylation.

    Purity of protein >90%.

  • Biological Activity - Recombinant mouse PD1 protein (Active) (ab180051), expandable thumbnail

    Biological Activity - Recombinant mouse PD1 protein (Active) (ab180051)

    Loaded Mouse PD-L1, Fc Tag on Protein A Biosensor, can bind Mouse PD-1, His Tag (ab180051) with an affinity constant of 6.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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