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AB271672

Recombinant mouse PDE1B protein (Active) (GST tag N-Terminus)

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Recombinant mouse PDE1B protein (Active) (GST tag N-Terminus) is a Mouse Fragment protein, in the 42 to 535 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Pde1b1, Pde1b, Cam-PDE 1B, 63 kDa Cam-PDE

2 Images
Functional Studies - Recombinant mouse PDE1B protein (Active) (GST tag N-Terminus) (AB271672)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse PDE1B protein (Active) (GST tag N-Terminus) (AB271672)

Specific activity of ab271672 was 5000 pmol/min/μg.

SDS-PAGE - Recombinant mouse PDE1B protein (Active) (GST tag N-Terminus) (AB271672)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse PDE1B protein (Active) (GST tag N-Terminus) (AB271672)

SDS-PAGE analysis of ab271672.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: 5000 pmol/min/μg.

Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.

Accession

Q01065

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Primary Antibodies

AB182565

Anti-PDE1B antibody [EPR12293(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE1B antibody [EPR12293(2)] (AB182565)

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  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"VKQLENGEVNIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWLASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVHNCLKNLDLWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPTVFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIEVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVFRMMQDDEMNIFINLTKDEFAELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADDDSKPKSQPSFQWRQPSLDVDVGDPNPDVVSFRATWTKYIQENKQKWKERAASGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD","proteinLength":"Fragment","predictedMolecularWeight":"83 kDa","actualMolecularWeight":null,"aminoAcidEnd":535,"aminoAcidStart":42,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q01065","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE1B also known as phosphodiesterase 1B is an enzyme that importantly regulates intracellular levels of cyclic nucleotides by hydrolyzing cAMP and cGMP. With a molecular mass of approximately 66 kDa PDE1B shows a widespread tissue distribution with significant expression in the brain heart and vascular smooth muscle. The enzyme functions as a calmodulin-dependent phosphodiesterase activating in the presence of calcium levels above basal.
Biological function summary

PDE1B plays an essential role in the signaling pathways that rely on cyclic nucleotides as second messengers. It regulates calcium-mediated signal transduction affecting activities of cells such as neurons and cardiac cells. While PDE1B primarily functions as a homodimer its activity interfaces with several other cellular processes through its regulation of cyclic nucleotide concentrations modulating downstream effects.

Pathways

PDE1B acts mainly in the cAMP and cGMP signaling pathways both of which are important for cellular responses to external stimuli. It modulates the degradation of these second messengers influencing associated proteins like protein kinase A (PKA) and protein kinase G (PKG). By controlling the half-life of cAMP and cGMP PDE1B plays an important role in signal cascades that govern processes such as learning memory and blood vessel relaxation.

PDE1B has associations with neurological and cardiovascular diseases. Abnormal expression of PDE1B can contribute to conditions such as schizophrenia and hypertension. Through its involvement in cyclic nucleotide signaling PDE1B is connected to proteins like D2 dopamine receptor in schizophrenia and angiotensin II receptors in hypertension where dysregulated signaling can impact disease progression and response to treatment.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Cyclic nucleotide phosphodiesterase with a dual specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.

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Product protocols

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Target data

Cyclic nucleotide phosphodiesterase with a dual specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.
See full target information Pde1b
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