Recombinant mouse PDE2A protein (Active) is a Mouse Full Length protein, in the 2 to 939 aa range, expressed in Baculovirus infected Sf9, with >=89% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>=89% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.Isoform PDE2A2Regulates mitochondrial cAMP levels and respiration (PubMed:21724846). Involved in the regulation of mitochondria morphology/dynamics and apoptotic cell death via local modulation of cAMP/PKA signaling in the mitochondrion, including the monitoring of local cAMP levels at the outer mitochondrial membrane and of PKA-dependent phosphorylation of DNM1L (PubMed:28463107).
Alternative names
Cyclic GMP-stimulated phosphodiesterase, CGS-PDE, cGSPDE, Pde2a
Recommended products
Recombinant mouse PDE2A protein (Active) is a Mouse Full Length protein, in the 2 to 939 aa range, expressed in Baculovirus infected Sf9, with >=89% purity and suitable for SDS-PAGE, FuncS.
Alternative names
Cyclic GMP-stimulated phosphodiesterase, CGS-PDE, cGSPDE, Pde2a
Key facts
- Purity
>=89% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Specific Activity: ≥20,000 pmole/min/μg.
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.
Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 μM cAMP, and serial dilutions of PDE2A at 37°C for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.- Accession
- Q922S4-1
- Animal free
No
- Species
Mouse
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% Glutathione, 0.04% Tween, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- Accession
- Protein length
Full Length
- Predicted molecular weight
130 kDa
- Amino acids
2 to 939
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.
- Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily.
- Subcellular localisation
Mitochondrion matrix, Mitochondrion inner membrane, Mitochondrion outer membrane
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- Activity summary
PDE2A or phosphodiesterase 2A is an enzyme involved in the breakdown of cyclic nucleotides specifically cAMP and cGMP through its phosphodiesterase activity. This protein has a mass of approximately 105 kDa and is also known as cGMP-stimulated phosphodiesterase. PDE2A is expressed in various tissues with the highest levels found in the brain heart and adrenal gland. It is essential in regulating intracellular signals making it an important player in cellular communication.
- Biological function summary
PDE2A plays a significant role in modulating signaling pathways by controlling the levels of cyclic nucleotides. It does not form part of a larger complex but interacts with other proteins to exert its function. Its activity impacts processes such as smooth muscle contraction neurotransmission and cardiac function. By hydrolyzing cAMP and cGMP PDE2A influences the cellular response to external stimuli and maintains homeostasis within cells.
- Pathways
Phosphodiesterase 2A significantly contributes to the cyclic nucleotide signaling pathway and the MAPK signaling pathway. By regulating cAMP and cGMP levels PDE2A influences signal transduction mechanisms linked to these pathways. It interfaces with proteins such as protein kinase A (PKA) and guanylyl cyclase affecting downstream responses that involve cell growth differentiation and apoptosis.
- Associated diseases and disorders
Abnormal PDE2A activity has implications in neurological and cardiovascular conditions. Dysregulation of PDE2A has been associated with depressive disorders and heart failure. Its relationship with other proteins such as PKA can alter neurotransmitter release in depression and affect cardiac muscle contractility in heart failure. These connections highlight PDE2A as a potential therapeutic target for managing such conditions.
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2 product images
Functional Studies - Recombinant mouse PDE2A protein (Active) (ab271675)
Specific activity of ab271675 was ≥20,000 pmole/min/μg.
SDS-PAGE - Recombinant mouse PDE2A protein (Active) (ab271675)
SDS-PAGE analysis of 1.9 μg ab271675.
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