Recombinant Mouse PDGF B Protein (Active) is a Mouse Full Length protein, in the 82 to 190 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
S L G S L A A A E P A V I A E C K T R T E V F Q I S R N L I D R T N A N F L V W P P C V E V Q R C S G C C N N R N V Q C R A S Q V Q M R P V Q V R K I E I V R K K P I F K K A T V T L E D H L A C K C E T I V T P R P V T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
Alternative names
Sis, Pdgfb, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis
Recommended products
Recombinant Mouse PDGF B Protein (Active) is a Mouse Full Length protein, in the 82 to 190 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent proliferation of Balb/c 3T3 cells. ED50 is ≤ 0.49 ng/ml, corresponding to a specific activity of 2.04 x 106 units/mg.
- Accession
- P31240-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- S L G S L A A A E P A V I A E C K T R T E V F Q I S R N L I D R T N A N F L V W P P C V E V Q R C S G C C N N R N V Q C R A S Q V Q M R P V Q V R K I E I V R K K P I F K K A T V T L E D H L A C K C E T I V T P R P V T
- Accession
- P31240
- Protein length
- Full Length
- Predicted molecular weight
- 12.28 kDa
- Actual molecular weight
- 12.28 kDa
- Amino acids
- 82 to 190
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
- Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
- Biological function summary
PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor β (PDGFR-β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.
- Pathways
PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.
- Associated diseases and disorders
Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.
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4 product images
Mass Spectrometry - Recombinant Mouse PDGF B Protein (Active) (ab307483)
Mass determination by ESI-TOF. Predicted MW is 12280.4 Da (+/-10 Da by ESI-TOF). Observed MW is 12280.44 Da.
SDS-PAGE - Recombinant Mouse PDGF B Protein (Active) (ab307483)
SDS-PAGE analysis of ab307483
HPLC - Recombinant Mouse PDGF B Protein (Active) (ab307483)
HPLC analysis of ab307483
Biological Activity - Recombinant Mouse PDGF B Protein (Active) (ab307483)
Fully biologically active determined by the dose dependent proliferation of Balb/c 3T3 cells. ED50 is ≤ 0.49 ng/ml, corresponding to a specific activity of 2.04 x 10^6 units/mg.
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