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AB289741

Recombinant Mouse PLD3 protein

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Recombinant Mouse PLD3 protein is a Mouse Full Length protein, in the 1 to 488 aa range, expressed in Cell free, with >85%, suitable for SDS-PAGE.

View Alternative Names

Sam9, Pld3, 5'-3' exonuclease PLD3, Choline phosphatase 3, Phosphatidylcholine-hydrolyzing phospholipase D3, Phospholipase D3, Schwannoma-associated protein 9, PLD 3, SAM-9

1 Images
SDS-PAGE - Recombinant Mouse PLD3 protein (AB289741)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse PLD3 protein (AB289741)

SDS-PAGE analysis of ab289741

Key facts

Purity

>85% SDS-PAGE

Expression system

Cell free

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O35405

Animal free

Yes

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MKPKLMYQELKVPVEEPAGELPLNEIEAWKAAEKKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGLEFPNATTSNPSTSQAWLGLLAGAHSSLDIASFYWTLTNNDTHTQEPSAQQGEEVLQQLQALAPRGVKVRIAVSKPNGPLADLQSLLQSGAQVRMVDMQKLTHGVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLARDLTKIFEAYWFLGQAGSSIPSTWPRSFDTRYNQETPMEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDSARSFIYIAVMNYLPTMEFSHPRRFWPAIDDGLRRAAYERGVKVRLLISCWGHSDPSMRSFLLSLAALHDNHTHSDIQVKLFVVPTDESQARIPYARVNHNKYMVTERASYIGTSNWSGSYFTETAGTSLLVTQNGHGGLRSQLEAVFLRDWESPYSHDLDTSANSVGNACRLL","proteinLength":"Full Length","predictedMolecularWeight":"58.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":488,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Cell free","accessionNumber":"O35405","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Lyophilized

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General info

Function

5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA) (PubMed : 30111894). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor in collaboration with PLD4 (PubMed : 30111894). May be important in myotube formation. Plays a role in lysosomal homeostasis. Involved in the regulation of endosomal protein sorting (By similarity).

Sequence similarities

Belongs to the phospholipase D family.

Post-translational modifications

N-glycosylated.. Proteolytically processed to a soluble form that is stable within endosomes and lysosomes. During transport through the secretory pathway becomes proteolysed by cysteine proteases, thereby releasing a stable soluble lysosomal lumenal polypeptide, whereas the transmembrane-bound fragment is rapidly degraded. Its transport route to lysosomes involves ubiquitination and the ESCRT complex.. Ubiquitinated. Ubiquitination mediates sorting into lysosomes.

Subcellular localisation

Lysosome lumen

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Product protocols

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Target data

5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA) (PubMed : 30111894). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor in collaboration with PLD4 (PubMed : 30111894). May be important in myotube formation. Plays a role in lysosomal homeostasis. Involved in the regulation of endosomal protein sorting (By similarity).
See full target information Pld3
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