Recombinant Mouse Protective protein/Cathepsin A (PPCA) (His tag) is a Mouse Full Length protein, in the 24 to 474 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A P D Q D E I D C L P G L A K Q P S F R Q Y S G Y L R A S D S K H F H Y W F V E S Q N D P K N S P V V L W L N G G P G C S S L D G L L T E H G P F L I Q P D G V T L E Y N P Y A W N L I A N V L Y I E S P A G V G F S Y S D D K M Y V T N D T E V A E N N Y E A L K D F F R L F P E Y K D N K L F L T G E S Y A G I Y I P T L A V L V M Q D P S M N L Q G L A V G N G L A S Y E Q N D N S L V Y F A Y Y H G L L G N R L W T S L Q T H C C A Q N K C N F Y D N K D P E C V N N L L E V S R I V G K S G L N I Y N L Y A P C A G G V P G R H R Y E D T L V V Q D F G N I F T R L P L K R R F P E A L M R S G D K V R L D P P C T N T T A P S N Y L N N P Y V R K A L H I P E S L P R W D M C N F L V N L Q Y R R L Y Q S M N S Q Y L K L L S S Q K Y Q I L L Y N G D V D M A C N F M G D E W F V D S L N Q K M E V Q R R P W L V D Y G E S G E Q V A G F V K E C S H I T F L T I K G A G H M V P T D K P R A A F T M F S R F L N K E P Y V E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
Alternative names
Ppgb, Ctsa, Lysosomal protective protein, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, Protective protein cathepsin A, Protective protein for beta-galactosidase, PPCA
Recommended products
Recombinant Mouse Protective protein/Cathepsin A (PPCA) (His tag) is a Mouse Full Length protein, in the 24 to 474 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P16675-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A P D Q D E I D C L P G L A K Q P S F R Q Y S G Y L R A S D S K H F H Y W F V E S Q N D P K N S P V V L W L N G G P G C S S L D G L L T E H G P F L I Q P D G V T L E Y N P Y A W N L I A N V L Y I E S P A G V G F S Y S D D K M Y V T N D T E V A E N N Y E A L K D F F R L F P E Y K D N K L F L T G E S Y A G I Y I P T L A V L V M Q D P S M N L Q G L A V G N G L A S Y E Q N D N S L V Y F A Y Y H G L L G N R L W T S L Q T H C C A Q N K C N F Y D N K D P E C V N N L L E V S R I V G K S G L N I Y N L Y A P C A G G V P G R H R Y E D T L V V Q D F G N I F T R L P L K R R F P E A L M R S G D K V R L D P P C T N T T A P S N Y L N N P Y V R K A L H I P E S L P R W D M C N F L V N L Q Y R R L Y Q S M N S Q Y L K L L S S Q K Y Q I L L Y N G D V D M A C N F M G D E W F V D S L N Q K M E V Q R R P W L V D Y G E S G E Q V A G F V K E C S H I T F L T I K G A G H M V P T D K P R A A F T M F S R F L N K E P Y V E H H H H H H
- Accession
- P16675
- Protein length
- Full Length
- Predicted molecular weight
- 52.4 kDa
- Amino acids
- 24 to 474
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
- Sequence similarities
Belongs to the peptidase S10 family.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protective protein/Cathepsin A (PPCA) also known as cathepsin A or protective proteins is an enzyme with a molecular mass of approximately 54 kDa. The enzyme is expressed widely in different tissues including liver kidney and the central nervous system. Mechanically PPCA plays a role as a carboxypeptidase with serine-type protease activity important for the stabilization and activation of lysosomal enzymes such as beta-galactosidase and neuraminidase. This stabilization is vital for proper lysosomal function and the breakdown of glycoproteins within the cell.
- Biological function summary
In conjunction with these enzymes PPCA forms part of the lysosomal multienzyme complex. It regulates the degradation of sialylated glycoconjugates by ensuring the structural integrity and enzymatic activity of lysosomal enzymes. This regulation prevents the accumulation of substrate materials which can disrupt cellular homeostasis. PPCA's interaction with beta-galactosidase and neuraminidase enables the proper catabolism of glycoproteins and glycolipids which is essential for cellular functions.
- Pathways
PPCA functions within the lysosomal storage and sialic acid catabolism pathways. These pathways are significant for the degradation and recycling of macromolecules in the lysosome maintaining cellular health. In the sialic acid metabolism pathway PPCA pairs with neuraminidase to break down sialylated substrates while interacting closely with beta-galactosidase in glycoprotein catabolism. These interactions highlight its role in preserving the efficiency of lysosomal degradation processes.
- Associated diseases and disorders
Mutations in the PPCA gene can lead to diseases like galactosialidosis and neuraminidase deficiency. Galactosialidosis arises from defective cathepsin A activity disrupting normal glycoprotein breakdown and resulting in storage disorders. This condition involves both beta-galactosidase and neuraminidase which accumulate as a result of PPCA dysfunction. Similarly neuraminidase deficiency exhibits symptoms due to impaired degradation of sialic acids indicating the necessity of PPCA in disease prevention.
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SDS-PAGE - Recombinant Mouse Protective protein/Cathepsin A (PPCA) (His tag) (ab222429)
15% SDS-PAGE analysis of 3 μg ab222429.
MWt 50-70 kDa (SDS-PAGE under reducing conditions).
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