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AB267856

Recombinant Mouse Pyruvate Dehydrogenase E2 protein (His tag)

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Recombinant Mouse Pyruvate Dehydrogenase E2 protein (His tag) is a Mouse Full Length protein, in the 86 to 642 aa range, expressed in Mammalian, with >90%, suitable for SDS-PAGE.

View Alternative Names

Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, Pyruvate dehydrogenase complex component E2, PDC-E2, PDCE2, Dlat

Key facts

Purity

>90% SDS-PAGE

Expression system

Mammalian

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8BMF4

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS, 6% Trehalose

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SLPPHQKVPLPSLSPTMQAGTIARWEKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKILVPEGTRDVPVGSIICITVEKPQDIEAFKNYTLDLAAAAAPQAAPAAAPAPAAAPAAPSASAPGSSYPTHMQIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGAPLCIIVEKQEDIAAFADYRPTEVTSLKPQAAPPAPPPVAAVPPTPQPVAPTPSAAPAGPKGRVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDIDSFVPSKAAPAAAAAMAPPGPRVAPAPAGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSVDVNMGEVLLVRKELNKMLEGKGKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVGAQWLAEFKKYLEKPITMLL","proteinLength":"Full Length","predictedMolecularWeight":"69 kDa","actualMolecularWeight":null,"aminoAcidEnd":642,"aminoAcidStart":86,"nature":"Recombinant","expressionSystem":"Mammalian","accessionNumber":"Q8BMF4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pyruvate Dehydrogenase E2 (PDH-E2) also referred to as PDC-E2 or E2 protein is a central component of cellular metabolism. It is the inner core dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex. PDH-E2 facilitates the conversion of pyruvate to acetyl-CoA which is an important step in aerobic respiration. The E2 protein is expressed in the mitochondria where it actively engages in its enzymatic roles. With a molecular weight of approximately 55 kDa PDH-E2 functions through its interaction with other components of the pyruvate dehydrogenase complex.
Biological function summary

PDH-E2 plays a significant role in cellular energy production. It is an important part of the pyruvate dehydrogenase complex where its coordinated enzymatic activity drives the decarboxylation of pyruvate. This activity supplies acetyl-CoA for the citric acid cycle essential for ATP production. PDH-E2's function regulates the flow of carbon within cells linking glycolysis and the citric acid cycle making it essential for energy homeostasis. Its function integrates signals that regulate metabolic pathways helping maintain cellular energy balance.

Pathways

PDH-E2 integrates the glycolysis and citric acid cycle facilitating efficient energy production. It interacts with tightly linked enzymes in the pyruvate dehydrogenase complex important for catalyzing the conversion processes. Key related proteins include pyruvate dehydrogenase (E1) and dihydrolipoamide dehydrogenase (E3) which form the complex alongside E2. This complex is also critical in adjusting metabolic responses based on cellular energy needs efficiently managing substrate flux into the mitochondria.

Dysfunctional PDH-E2 activity associates with metabolic conditions like pyruvate dehydrogenase deficiency and primary biliary cholangitis (PBC). PDH deficiency leads to lactic acidosis and neurological dysfunction due to impaired energy production. In PBC the immune system aberrantly targets the E2 component causing liver damage and leading to cholestasis. These diseases highlight the critical role of PDH-E2 and its interaction with other mitochondrial proteins in maintaining metabolic health.
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Specifications

Form

Liquid

Additional notes

nan

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General info

Function

As part of the pyruvate dehydrogenase complex, catalyzes the transfers of an acetyl group to a lipoic acid moiety. The pyruvate dehydrogenase complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle.

Sequence similarities

Belongs to the 2-oxoacid dehydrogenase family.

Post-translational modifications

Delipoylated at Lys-131 and Lys-258 by SIRT4, delipoylation decreases the PHD complex activity.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

As part of the pyruvate dehydrogenase complex, catalyzes the transfers of an acetyl group to a lipoic acid moiety. The pyruvate dehydrogenase complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle.
See full target information Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
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Complementary Products

Secondary Antibodies

AB150113

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB172617

Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]

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Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (AB172617)

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Proteins & Peptides

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Multiplex Kits

AB228525

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Recombinant

Western blot - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

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