Recombinant Mouse RANKL Protein (Active) is a Mouse Fragment protein, in the 141 to 316 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, FuncS, Cell Culture, SDS-PAGE, MS.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, FuncS, Cell Culture, SDS-PAGE, MS
- Biologically active
- Yes
Amino acid sequence
G A P A M M E G S W L D V A Q R G K P E A Q P F A H L T I N A A S I P S G S H K V T L S S W Y H D R G W A K I S N M T L S N G K L R V N Q D G F Y Y L Y A N I C F R H H E T S G S V P T D Y L Q L M V Y V V K T S I K I P S S H N L M K G G S T K N W S G N S E F H F Y S I N V G G F F K L R A G E E I S I Q V S N P S L L D P D Q D A T Y F G A F K V Q D I D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor (PubMed:22437732). Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (By similarity). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts (PubMed:18586671, PubMed:24039232, PubMed:27336669). During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (PubMed:23395171, PubMed:26644563).
Alternative names
CD254, Opgl, Rankl, Trance, Tnfsf11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE
Recommended products
Recombinant Mouse RANKL Protein (Active) is a Mouse Fragment protein, in the 141 to 316 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, FuncS, Cell Culture, SDS-PAGE, MS.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, FuncS, Cell Culture, SDS-PAGE, MS
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent NF-ΚB activation of mouse RAW 264.7 macrophages overexpressing AP-1 and NF-ΚB inducible SEAP reporter gene. Results obtained with InvivoGen RAW Blue™ cells (Cat code.,raw-sp). ED50 ≤ 207 ng/ml, corresponding to a specific activity of 4.8 x 10^3 units/mg.
- Accession
- O35235-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- G A P A M M E G S W L D V A Q R G K P E A Q P F A H L T I N A A S I P S G S H K V T L S S W Y H D R G W A K I S N M T L S N G K L R V N Q D G F Y Y L Y A N I C F R H H E T S G S V P T D Y L Q L M V Y V V K T S I K I P S S H N L M K G G S T K N W S G N S E F H F Y S I N V G G F F K L R A G E E I S I Q V S N P S L L D P D Q D A T Y F G A F K V Q D I D
- Accession
- O35235
- Protein length
- Fragment
- Predicted molecular weight
- 19.54 kDa
- Actual molecular weight
- 19.54 kDa
- Amino acids
- 141 to 316
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor (PubMed:22437732). Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (By similarity). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts (PubMed:18586671, PubMed:24039232, PubMed:27336669). During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (PubMed:23395171, PubMed:26644563).
- Sequence similarities
Belongs to the tumor necrosis factor family.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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3 product images
Biological Activity - Recombinant Mouse RANKL Protein (Active) (ab307187)
Fully biologically active determined by the dose dependent NF-ΚB activation of mouse RAW 264.7 macrophages overexpressing AP-1 and NF-ΚB inducible SEAP reporter gene. Results obtained with Invivogen RAW Blue™ cells (Cat code.,raw-sp). ED50 ≤ 207 ng/ml, corresponding to a specific activity of 4.8 x 103 units/mg.
Mass Spectrometry - Recombinant Mouse RANKL Protein (Active) (ab307187)
Mass determination by ESI-TOF.
Predicted MW is 19539.97 Da (+/- 10 Da by ESI-TOF). Observed MW is 19541.33 Da.
SDS-PAGE - Recombinant Mouse RANKL Protein (Active) (ab307187)
SDS-page analysis of ab307187
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