Recombinant Mouse Robo4 protein (His tag) is a Mouse Fragment protein, in the 1 to 480 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S G G T G L L G T E W P L P L L L L F I M G G E A L D S P P Q I L V H P Q D Q L L Q G S G P A K M R C R S S G Q P P P T I R W L L N G Q P L S M A T P D L H Y L L P D G T L L L H R P S V Q G R P Q D D Q N I L S A I L G V Y T C E A S N R L G T A V S R G A R L S V A V L Q E D F Q I Q P R D T V A V V G E S L V L E C G P P W G Y P K P S V S W W K D G K P L V L Q P G R R T V S G D S L M V S R A E K N D S G T Y M C M A T N N A G Q R E S R A A R V S I Q E S Q D H K E H L E L L A V R I Q L E N V T L L N P E P V K G P K P G P S V W L S W K V S G P A A P A E S Y T A L F R T Q R S P R D Q G S P W T E V L L R G L Q S A K L G G L H W G Q D Y E F K V R P S S G R A R G P D S N V L L L R L P E Q V P S A P P Q G V T L R S G N G S V F V S W A P P P A E S H N G V I R G Y Q V W S L G N A S L P A A N W T V V G E Q T Q L E I A T R L P G S Y C V Q V A A V T G A G A G E L S T P V C L L L E Q A M E Q S A R D P R K H V P W T L E Q L R A T L R R P E V I A S S A V L L W
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Involved in the maintenance of endothelial barrier organization and function (By similarity).
Alternative names
Roundabout homolog 4, Robo4
Recommended products
Recombinant Mouse Robo4 protein (His tag) is a Mouse Fragment protein, in the 1 to 480 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8C310-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M G S G G T G L L G T E W P L P L L L L F I M G G E A L D S P P Q I L V H P Q D Q L L Q G S G P A K M R C R S S G Q P P P T I R W L L N G Q P L S M A T P D L H Y L L P D G T L L L H R P S V Q G R P Q D D Q N I L S A I L G V Y T C E A S N R L G T A V S R G A R L S V A V L Q E D F Q I Q P R D T V A V V G E S L V L E C G P P W G Y P K P S V S W W K D G K P L V L Q P G R R T V S G D S L M V S R A E K N D S G T Y M C M A T N N A G Q R E S R A A R V S I Q E S Q D H K E H L E L L A V R I Q L E N V T L L N P E P V K G P K P G P S V W L S W K V S G P A A P A E S Y T A L F R T Q R S P R D Q G S P W T E V L L R G L Q S A K L G G L H W G Q D Y E F K V R P S S G R A R G P D S N V L L L R L P E Q V P S A P P Q G V T L R S G N G S V F V S W A P P P A E S H N G V I R G Y Q V W S L G N A S L P A A N W T V V G E Q T Q L E I A T R L P G S Y C V Q V A A V T G A G A G E L S T P V C L L L E Q A M E Q S A R D P R K H V P W T L E Q L R A T L R R P E V I A S S A V L L W
- Accession
- Q8C310
- Protein length
- Fragment
- Predicted molecular weight
- 49.6 kDa
- Amino acids
- 1 to 480
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Involved in the maintenance of endothelial barrier organization and function (By similarity).
- Sequence similarities
Belongs to the immunoglobulin superfamily. ROBO family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Roundabout homolog 4 (Robo4) is a protein weighing approximately 107 kDa. It belongs to the immunoglobulin superfamily and is sometimes referred to as Magic Roundabout. Robo4 primarily gets expressed in endothelial cells playing an important function in vascular biology. It is characterized by having two immunoglobulin-like domains and two fibronectin type III domains making it distinct among the other Robo family members.
- Biological function summary
Robo4 stabilizes the vascular network interacting actively with the guidance molecule Slit proteins. It is not part of a larger protein complex but engages directly with these ligands to influence endothelial cell migration and vascular permeability. Its role is significant in maintaining the structural integrity of blood vessels ensuring the appropriate cellular responses important for proper vascular function.
- Pathways
Robo4 engages in the Slit-Robo signaling pathway that is essential for vascular development and remodeling. This pathway helps inhibit excessive endothelial migration and angiogenesis. Robo4 interacts with proteins like Slit2 which binds to it as a ligand to mediate these effects. Through this pathway Robo4 performs an important function in processes such as vascular homeostasis and angiogenesis regulation.
- Associated diseases and disorders
Robo4 appears to have connections to vascular-related conditions including diabetic retinopathy and age-related macular degeneration. The protein influences pathological angiogenesis which is a hallmark in the progression of these diseases. In these contexts Robo4's interaction with Slit2 proves important as it can restrain abnormal vascular growth offering potential therapeutic angles for treatment approaches focused on targeting the Slit-Robo pathway.
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1 product image
SDS-PAGE - Recombinant Mouse Robo4 protein (His tag) (ab276973)
SDS-PAGE analysis of ab276973
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