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AB214826

Recombinant Mouse SEMA4A protein (Fc Chimera)

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Recombinant Mouse SEMA4A protein (Fc Chimera) is a Mouse Fragment protein, in the 1 to 676 aa range, expressed in CHO cells, with >98%, < 0.06 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Semab, SemB, Sema4a, Semaphorin-4A, Semaphorin-B, Sema B

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 0.06 EU/µg

Expression system

CHO cells

Tags

Fc tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q62178

Animal free

No

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute at 100 µg/mL in PBS

Storage buffer

Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MALPSLGQDSWSLLRVFFFQLFLLPSLPPASGTGGQGPMPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSDDGNTLYVGAREAVLALNIQNPGIPRLKNMIPWPASERKKTECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSLLLPILIDKVMDGKGQSPFDPVHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHADASFVAAIPSTQVVYFFFEETASEFDFFEELYISRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVSRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVSPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGTPLLVKSGVEYTRLAVESARGLDGSSHVVMYLGTSTGSLHKAVVPQDSSAYLVEEIQLSPDSEPVRNLQLAPAQGAVFAGFSGGIWRVPRANCSVYESCVDCVLARDPHCAWDPESRLCSLLSGSTKPWKQDMERGNPEWVCTRGPMARSPRRQSPPQLIKEVLTVPNSILELPCPHLSALASYHWSHGRAKISEASATVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQ","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":676,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"CHO cells","accessionNumber":"Q62178","tags":[{"tag":"Fc","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEMA4A also known as Semaphorin-4A is a protein that functions as a guidance cue in the immune and nervous systems. It has a molecular mass of approximately 85 kDa. SEMA4A is expressed in various tissues including the retina lung and thymus as well as in several immune cells such as T cells B cells and dendritic cells. This protein plays a significant role in cell signaling affecting cellular processes like axonal guidance and immune responses.
Biological function summary

The interaction of SEMA4A with its receptors such as Plexin B1 and TIM-2 mediates important cellular activities. It is part of the broader semaphorin family which has a role in directing axonal growth during development. This protein contributes to the regulation of immune cell activity including the activation and differentiation of T cells. The presence of SEMA4A influences immune responses highlighting its importance in maintaining immune system balance.

Pathways

SEMA4A engages in the immune system and axon guidance pathways. In the immune system it interacts with proteins like Plexin B1 and CD72 influencing T cell proliferation and function. Through the axon guidance pathway SEMA4A helps direct the growth of nerve fibers impacting neuronal circuit formation. These interactions exemplify SEMA4A's role in linking cell signaling with physiological outcomes.

SEMA4A has connections with retinal degenerative diseases and immune system disorders. Mutations in the SEMA4A gene have been associated with retinitis pigmentosa a retinal disorder leading to vision loss. SEMA4A also plays a role in autoimmune diseases where its interaction with receptors like Plexin B1 affects immune cell function. Understanding the involvement of SEMA4A in these conditions helps in developing potential therapeutic approaches targeting its signaling pathways.
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Specifications

Form

Lyophilized

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General info

Function

Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling (PubMed : 17318185, PubMed : 20043131). Regulates glutamatergic and GABAergic synapse development (PubMed : 29981480). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner and promotes the development of excitatory synapses in a PLXNB2-dependent manner (PubMed : 29981480). Plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity (PubMed : 15780988). Promotes phosphorylation of TIMD2 (PubMed : 12374982). Inhibits angiogenesis (PubMed : 17318185). Promotes axon growth cone collapse (PubMed : 20043131). Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons (PubMed : 20043131).

Sequence similarities

Belongs to the semaphorin family.

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Product protocols

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Target data

Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling (PubMed : 17318185, PubMed : 20043131). Regulates glutamatergic and GABAergic synapse development (PubMed : 29981480). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner and promotes the development of excitatory synapses in a PLXNB2-dependent manner (PubMed : 29981480). Plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity (PubMed : 15780988). Promotes phosphorylation of TIMD2 (PubMed : 12374982). Inhibits angiogenesis (PubMed : 17318185). Promotes axon growth cone collapse (PubMed : 20043131). Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons (PubMed : 20043131).
See full target information Sema4a
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