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AB313359

Recombinant Mouse SERPINA1A Protein

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Recombinant Mouse SERPINA1A Protein is a Mouse Full Length protein, in the 25 to 413 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, Mass Spec.

View Alternative Names

Dom1, Spi1-1, Serpina1a, Alpha-1-antitrypsin 1-1, AAT, Alpha-1 protease inhibitor 1, Alpha-1-antiproteinase, Serine protease inhibitor 1-1, Serine protease inhibitor A1a, Serpin A1a

3 Images
Mass Spectrometry - Recombinant Mouse SERPINA1A Protein (AB313359)
  • Mass Spec

Lab

Mass Spectrometry - Recombinant Mouse SERPINA1A Protein (AB313359)

Mass determination by ESI-TOF. Predicted MW is 43571.91(+/- 10Da by ESI-TOF). Observed MW is 43575.37.

HPLC - Recombinant Mouse SERPINA1A Protein (AB313359)
  • HPLC

Lab

HPLC - Recombinant Mouse SERPINA1A Protein (AB313359)

HPLC analysis of ab313359

SDS-PAGE - Recombinant Mouse SERPINA1A Protein (AB313359)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse SERPINA1A Protein (AB313359)

SDS-page analysis of ab313359

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, SDS-PAGE, HPLC

applications

Biologically active

No

Accession

P07758

Animal free

Yes

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"EDVQETDTSQKDQSPASHEIATNLGDFAISLYRELVHQSNTSNIFFSPVSIATAFAMLSLGSKGDTHTQILEGLQFNLTQTSEADIHKSFQHLLQTLNRPDSELQLSTGNGLFVNNDLKLVEKFLEEAKNHYQAEVFSVNFAESEEAKKVINDFVEKGTQGKIAEAVKKLDQDTVFALANYILFKGKWKKPFDPENTEEAEFHVDESTTVKVPMMTLSGMLHVHHCSTLSSWVLLMDYAGNATAVFLLPDDGKMQHLEQTLSKELISKFLLNRRRRLAQIHFPRLSISGEYNLKTLMSPLGITRIFNNGADLSGITEENAPLKLSQAVHKAVLTIDETGTEAAAVTVLQMVPMSMPPILRFDHPFLFIIFEEHTQSPIFLGKVVDPTHK","proteinLength":"Full Length","predictedMolecularWeight":"43.6 kDa","actualMolecularWeight":"43.6 kDa","aminoAcidEnd":413,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P07758","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.
Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.
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Specifications

Form

Lyophilized

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General info

Function

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.

Sequence similarities

Belongs to the serpin family.

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Product protocols

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Target data

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.
See full target information Serpina1a
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