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Recombinant Mouse SP-D Protein Standard (His tag) is a Mouse Full Length protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.

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Images

SDS-PAGE - Recombinant Mouse SP-D Protein Standard (His tag) (AB316638), expandable thumbnail
  • Sandwich ELISA - Recombinant Mouse SP-D Protein Standard (His tag) (AB316638), expandable thumbnail

Key facts

Purity
>=80% SDS-PAGE
Expression system
HEK 293 cells
Tags
His tag C-Terminus
Applications
sELISA, SDS-PAGE
Biologically active
No

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Alternative names

Recommended products

Recombinant Mouse SP-D Protein Standard (His tag) is a Mouse Full Length protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.

Key facts

Purity
>=80% SDS-PAGE
Expression system
HEK 293 cells
Applications
sELISA, SDS-PAGE
Accession
P50404-1
Animal free
Yes
Species
Mouse
Concentration
Loading...
Storage buffer

pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Accession
P50404
Protein length
Full Length
Predicted molecular weight
37.2 kDa
Nature
Recombinant
Tags
His tag C-Terminus

Specifications

Form
Liquid

General info

Function

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Sequence similarities

Belongs to the SFTPD family.

Post-translational modifications

S-nitrosylation at Cys-34 and Cys-39 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Surfactant protein D (SP-D) also known as protein D functions as an important component of the innate immune system. It weighs around 43 kDa and is primarily expressed in pulmonary alveoli where lung cells synthesize it. SP-D belongs to the collectin family and has a C-type lectin domain that allows binding to carbohydrate structures on microbial surfaces. This ability enables SP-D to interact directly with various pathogens and infected cells targeting them for clearance. Researchers often study SP-D with 'anti-surfactant' antibodies using methods like the ELISA protocol including versions like ELISA Sp and ELISA Denise.

Biological function summary

SP-D contributes to host defense by enhancing the uptake and elimination of pathogens by immune cells such as macrophages. Part of a larger protein complex SP-D can opsonize bacteria and viruses facilitating phagocytosis. Its role extends to modulating inflammation by affecting the production of pro-inflammatory cytokines. By maintaining homeostasis in surfactant composition it indirectly supports lung function and protects against lung injury. SP-D’s function involves communication and transmission of signals within the immune system making it integral to pulmonary immune processes.

Pathways

Surfactant protein D is involved in the regulation of the complement and coagulation cascades as well as the pathogen recognition pathway. It is closely related to other collectins such as surfactant protein A (SP-A) which share similar mechanisms in the immune response. SP-D's interaction within these pathways highlights its role in identifying and neutralizing pathogens. Through its connections with related proteins SP-D plays an important part in modulating immune responses ensuring effective pathogen elimination without excessive inflammation.

Associated diseases and disorders

SP-D has been linked with respiratory diseases such as chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis. These conditions may involve impaired SP-D function or expression levels potentially due to genetic factors or environmental influences. SP-D also interacts with proteins like SP-A in these diseases participating in lung immune responses. Disrupted regulation of SP-D can contribute to chronic inflammatory states in the lungs exacerbating disease progression and leading to compromised lung function. Understanding SP-D's role in these contexts offers potential therapeutic insights for targeting pathological inflammation.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Recombinant Mouse SP-D Protein Standard (His tag) (ab316638), expandable thumbnail

    SDS-PAGE - Recombinant Mouse SP-D Protein Standard (His tag) (ab316638)

    SDS-PAGE analysis of ab316638 under reducing conditions for 2ug protein.

  • Sandwich ELISA - Recombinant Mouse SP-D Protein Standard (His tag) (ab316638), expandable thumbnail

    Sandwich ELISA - Recombinant Mouse SP-D Protein Standard (His tag) (ab316638)

    Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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