Recombinant Mouse sPLA2-IIE protein (His tag) is a Mouse Full Length protein, in the 1 to 142 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M K P P I A L A C L C L L V P L A G G N L V Q F G V M I E R M T G K P A L Q Y N D Y G C Y C G V G G S H W P V D E T D W C C H A H D C C Y G R L E K L G C D P K L E K Y L F S I T R D N I F C A G R T A C Q R H T C E C D K R A A L C F R H N L N T Y N R K Y A H Y P N K L C T G P T P P C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids (PubMed:10531313, PubMed:11922621). Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity), releasing various unsaturated fatty acids including oleoate, linoleoate, arachidonate, docosahexaenoate and lysophosphatidylethanolamines in preference to lysophosphatidylcholines (By similarity). In response to high-fat diet, hydrolyzes minor lipoprotein phospholipids including phosphatidylserines, phosphatidylinositols and phosphatidylglycerols, altering lipoprotein composition and fat storage in adipose tissue and liver (PubMed:24910243). May act in an autocrine and paracrine manner (By similarity). Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Cleaves sn-2 fatty acyl chains of phosphatidylglycerols and phosphatidylethanolamines, which are major components of membrane phospholipids in bacteria (By similarity). Acts as a hair follicle phospholipase A2. Selectively releases lysophosphatidylethanolamines (LPE) and various unsaturated fatty acids in skin to regulate hair follicle homeostasis (PubMed:27226633). May regulate the inflammatory response by releasing arachidonate, a precursor of prostaglandins and leukotrienes. Upon allergen exposure, may participate in allergic inflammatory response by enhancing leukotriene C4 synthesis and degranulation in mast cells (PubMed:11922621).
Alternative names
Group IIE secretory phospholipase A2, GIIE sPLA2, sPLA2-IIE, Phosphatidylcholine 2-acylhydrolase 2E, Pla2g2e
Recommended products
Recombinant Mouse sPLA2-IIE protein (His tag) is a Mouse Full Length protein, in the 1 to 142 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9QUL3-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M K P P I A L A C L C L L V P L A G G N L V Q F G V M I E R M T G K P A L Q Y N D Y G C Y C G V G G S H W P V D E T D W C C H A H D C C Y G R L E K L G C D P K L E K Y L F S I T R D N I F C A G R T A C Q R H T C E C D K R A A L C F R H N L N T Y N R K Y A H Y P N K L C T G P T P P C
- Accession
- Q9QUL3
- Protein length
- Full Length
- Predicted molecular weight
- 15.5 kDa
- Amino acids
- 1 to 142
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids (PubMed:10531313, PubMed:11922621). Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity), releasing various unsaturated fatty acids including oleoate, linoleoate, arachidonate, docosahexaenoate and lysophosphatidylethanolamines in preference to lysophosphatidylcholines (By similarity). In response to high-fat diet, hydrolyzes minor lipoprotein phospholipids including phosphatidylserines, phosphatidylinositols and phosphatidylglycerols, altering lipoprotein composition and fat storage in adipose tissue and liver (PubMed:24910243). May act in an autocrine and paracrine manner (By similarity). Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Cleaves sn-2 fatty acyl chains of phosphatidylglycerols and phosphatidylethanolamines, which are major components of membrane phospholipids in bacteria (By similarity). Acts as a hair follicle phospholipase A2. Selectively releases lysophosphatidylethanolamines (LPE) and various unsaturated fatty acids in skin to regulate hair follicle homeostasis (PubMed:27226633). May regulate the inflammatory response by releasing arachidonate, a precursor of prostaglandins and leukotrienes. Upon allergen exposure, may participate in allergic inflammatory response by enhancing leukotriene C4 synthesis and degranulation in mast cells (PubMed:11922621).
- Sequence similarities
Belongs to the phospholipase A2 family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Secretory phospholipase A2 group IIE (sPLA2-IIE) is an enzyme belonging to the phospholipase A2 family also known as PLA2G2E. It has a molecular mass of approximately 14 kDa. sPLA2-IIE hydrolyzes the sn-2 position of glycerophospholipids releasing free fatty acids and lysophospholipids. This enzyme shows expression in various tissues including the pancreas and inflammatory sites where it contributes to lipid metabolism and cell membrane remodeling.
- Biological function summary
Secretory phospholipase A2 group IIE plays a role in inflammation and immune responses. Its activity results in the production of arachidonic acid and lysophospholipids which are precursors for eicosanoids like prostaglandins and leukotrienes. These lipid mediators regulate inflammatory processes thereby altering cellular responses. sPLA2-IIE participates in a complex network of enzymes and receptors modulating lipid signaling and affecting diverse physiological functions.
- Pathways
SPLA2-IIE interacts within the arachidonic acid pathway and the eicosanoid biosynthesis pathway. In these pathways sPLA2-IIE generates bioactive lipids impacting inflammatory signaling. It functions alongside proteins such as cyclooxygenase (COX) and lipoxygenase (LOX) which further metabolize arachidonic acid into specific eicosanoids. These pathways illustrate the enzyme's contribution to finely tuning the inflammatory response and cellular signaling networks.
- Associated diseases and disorders
Secretory phospholipase A2 group IIE associates with conditions like rheumatoid arthritis and atherosclerosis. Its enzymatic activity correlates with the inflammatory components of these diseases promoting tissue damage and disease progression. Through its involvement in lipid mediator synthesis sPLA2-IIE interacts with proteins like interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) key players in the inflammatory pathways that drive these disorders. Understanding the role of sPLA2-IIE in these diseases can help develop targeted therapies.
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1 product image
SDS-PAGE - Recombinant Mouse sPLA2-IIE protein (His tag) (ab276900)
SDS-PAGE analysis of ab276900
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