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AB277023

Recombinant Mouse SPLASH protein (Fc Chimera)

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Recombinant Mouse SPLASH protein (Fc Chimera) is a Mouse Full Length protein, in the 1 to 144 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Pla2a2, Splash, Pla2g2d, Group IID secretory phospholipase A2, GIID sPLA2, sPLA2-IID, PLA2IID, Phosphatidylcholine 2-acylhydrolase 2D

1 Images
SDS-PAGE - Recombinant Mouse SPLASH protein (Fc Chimera) (AB277023)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse SPLASH protein (Fc Chimera) (AB277023)

SDS-PAGE analysis of ab277023

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9WVF6

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

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Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB47118

Anti-SPLASH antibody

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Secondary Antibodies

AB150155

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

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Primary Antibodies

AB185014

Alexa Fluor® 488 Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB185014)

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Primary Antibodies

AB133270

Anti-Monoamine Oxidase B/MAOB antibody [EPR7102]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (AB133270)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRLALLCGLLLAGITATQGGLLNLNKMVTHMTGKKAFFSYWPYGCHCGLGGKGQPKDATDWCCQKHDCCYAHLKIDGCKSLTDNYKYSISQGTIQCSDNGSWCERQLCACDKEVALCLKQNLDSYNKRLRYYWRPRCKGKTPAC","proteinLength":"Full Length","predictedMolecularWeight":"41 kDa","actualMolecularWeight":null,"aminoAcidEnd":144,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9WVF6","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SPLASH protein also known as Synapse Protein LL Alpha SH3 functions as an adaptor in synaptic signaling. A molecular weight of approximately 50 kDa characterizes SPLASH and it shows high expression in neural tissues notably within the brain's synaptic regions. Synapse formation and plasticity strongly depend on the presence and function of this protein. It interacts with multiple synaptic components to assist in the organization of signal transduction complexes.
Biological function summary

The SPLASH protein coordinates with other synaptic proteins to facilitate neurotransmitter release and receptor clustering at the synapse. As part of larger protein complexes SPLASH forms dynamic interactions important for maintaining synaptic strength and plasticity. These complexes modulate synaptic vesicle exocytosis and endocytosis processes which are essential for efficient neuronal communication.

Pathways

The SPLASH protein integrates within the synaptic signaling pathway and the calcium signaling pathway. It serves as a link between the two pathways coordinating signal transduction events that modulate synaptic plasticity. SPLASH maintains functional connection with proteins like SNARE and PSD-95 which play central roles in synaptic vesicle fusion and postsynaptic density organization respectively.

The SPLASH protein links to neurodevelopmental disorders and synaptic dysfunction. It is particularly associated with conditions such as autism spectrum disorder where synaptic signaling and plasticity are affected. Moreover in Alzheimer's disease altered SPLASH expression influences amyloid precursor protein processing through its interactions with the Tau protein impacting cognitive function and neuronal integrity.
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Specifications

Form

Lyophilized

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General info

Function

Secretory calcium-dependent phospholipase A2 that primarily targets extracellular lipids, exerting anti-inflammatory and immunosuppressive functions (PubMed : 10455175, PubMed : 10531313, PubMed : 23690440, PubMed : 26392224). Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines (By similarity). In draining lymph nodes, selectively hydrolyzes diacyl and alkenyl forms of phosphatidylethanolamines, releasing omega-3 polyunsaturated fatty acids (PUFAs) such as eicosapentaenoate and docosahexaenoate that are precursors of the anti-inflammatory lipid mediators, resolvins (PubMed : 23690440). During the resolution phase of acute inflammation drives docosahexaenoate-derived resolvin D1 synthesis, which suppresses dendritic cell activation and T-helper 1 immune response (PubMed : 23690440, PubMed : 27226632). May act in an autocrine and paracrine manner. Via a mechanism independent of its catalytic activity, promotes differentiation of regulatory T cells (Tregs) and participates in the maintenance of immune tolerance (PubMed : 19564598). May contribute to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane (PubMed : 11694541).

Sequence similarities

Belongs to the phospholipase A2 family.

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Product protocols

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Target data

Secretory calcium-dependent phospholipase A2 that primarily targets extracellular lipids, exerting anti-inflammatory and immunosuppressive functions (PubMed : 10455175, PubMed : 10531313, PubMed : 23690440, PubMed : 26392224). Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines (By similarity). In draining lymph nodes, selectively hydrolyzes diacyl and alkenyl forms of phosphatidylethanolamines, releasing omega-3 polyunsaturated fatty acids (PUFAs) such as eicosapentaenoate and docosahexaenoate that are precursors of the anti-inflammatory lipid mediators, resolvins (PubMed : 23690440). During the resolution phase of acute inflammation drives docosahexaenoate-derived resolvin D1 synthesis, which suppresses dendritic cell activation and T-helper 1 immune response (PubMed : 23690440, PubMed : 27226632). May act in an autocrine and paracrine manner. Via a mechanism independent of its catalytic activity, promotes differentiation of regulatory T cells (Tregs) and participates in the maintenance of immune tolerance (PubMed : 19564598). May contribute to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane (PubMed : 11694541).
See full target information Pla2g2d
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