Recombinant Mouse ST6Gal1 Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.
Images
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, sELISA
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.
Alternative names
Siat1, St6gal1, ST6Gal I, Sialyltransferase 1, ST6GalI
Recombinant Mouse ST6Gal1 Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, sELISA
- Biologically active
- No
- Accession
- Q64685-1
- Animal free
- Yes
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Accession
- Q64685
- Protein length
- Fragment
- Predicted molecular weight
- 43.4 kDa
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.
- Sequence similarities
Belongs to the glycosyltransferase 29 family.
- Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD75 also known as ST6GAL1 is an enzyme that belongs to the glycosyltransferase family specifically classified as a sialyltransferase. CD75 catalyzes the addition of sialic acid to glycoproteins and glycolipids impacting their function and stability. It has an approximate molecular mass of 45 kDa. CD75 is expressed in a variety of cell types including B lymphocytes and epithelial cells. Its expression can be observed on the cell surface where it plays important roles in cell-cell interactions.
- Biological function summary
CD75 contributes to the mechanics of cell signaling and immune system modulation. CD75 is involved in the biosynthesis of sialylated glycoconjugates which affect cellular recognition and communication. This enzyme does not form part of a multi-protein complex but interacts with other proteins upon glycosylation events. Through its action CD75 impacts multiple cellular processes including inflammation regulation and pathogen recognition.
- Pathways
CD75 plays roles in glycosylation and immune response mechanisms. It participates in the N-glycan biosynthesis pathway which influences protein folding and stability. Additionally CD75 impacts the sialylation pathway where it modifies the surface proteins for lymphocyte activation. Importantly CD75 interacts with proteins such as Siglecs which are involved in immune response modulation and cellular recognition.
- Associated diseases and disorders
CD75 is implicated in cancer progression and autoimmune diseases. In various cancers such as colon cancer altered CD75 expression affects metastatic potential and tumor cell survival. Additionally aberrant CD75 expression occurs in autoimmune diseases like lupus wherein it modulates immune cell activity. CD75 interacts with proteins like CD22 a sialic acid-binding lectin on B cells influencing disease pathogenesis and progression through immune system pathways.
Product promise
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2 product images
Sandwich ELISA - Recombinant Mouse ST6Gal1 Protein Standard (His tag) (ab316715)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
SDS-PAGE - Recombinant Mouse ST6Gal1 Protein Standard (His tag) (ab316715)
SDS-PAGE analysis of ab316715 under reducing conditions for 2ug protein.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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