Recombinant Mouse STIP1/STI1 protein (His tag) is a Mouse Full Length protein, in the 1 to 543 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M E Q V N E L K E K G N K A L S A G N I D D A L Q C Y S E A I K L D P Q N H V L Y S N R S A A Y A K K G D Y Q K A Y E D G C K T V D L K P D W G K G Y S R K A A A L E F L N R F E E A K R T Y E E G L K H E A N N L Q L K E G L Q N M E A R L A E R K F M N P F N L P N L Y Q K L E N D P R T R S L L S D P T Y R E L I E Q L Q N K P S D L G T K L Q D P R V M T T L S V L L G V D L G S M D E E E E A A T P P P P P P P K K E P K P E P M E E D L P E N K K Q A L K E K E L G N D A Y K K K D F D K A L K H Y D R A K E L D P T N M T Y I T N Q A A V H F E K G D Y N K C R E L C E K A I E V G R E N R E D Y R Q I A K A Y A R I G N S Y F K E E K Y K D A H F Y N K S L A E H R T P D V L K K C Q Q A E K I L K E Q E R L A Y I N P D L A L E E K N K G N E C F Q K G D Y P Q A M K H Y T E A I K R N P R D A K L Y S N R A A C Y T K L L E F Q L A L K D C E E C I Q L E P T F I K G Y T R K A A A L E A M K D Y T K A M D V Y Q K A L D L D S S C K E A A D G Y Q R C M M A Q Y N R H D S P E D V K R R A M A D P E V Q Q I M S D P A M R L I L E Q M Q K D P Q A L S E H L K N P V I A Q K I Q K L M D V G L I A I R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acts as a co-chaperone for HSP90AA1. Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90.
Alternative names
Stress-induced-phosphoprotein 1, STI1, mSTI1, Hsc70/Hsp90-organizing protein, Hop, Stip1
Recommended products
Recombinant Mouse STIP1/STI1 protein (His tag) is a Mouse Full Length protein, in the 1 to 543 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q60864-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M E Q V N E L K E K G N K A L S A G N I D D A L Q C Y S E A I K L D P Q N H V L Y S N R S A A Y A K K G D Y Q K A Y E D G C K T V D L K P D W G K G Y S R K A A A L E F L N R F E E A K R T Y E E G L K H E A N N L Q L K E G L Q N M E A R L A E R K F M N P F N L P N L Y Q K L E N D P R T R S L L S D P T Y R E L I E Q L Q N K P S D L G T K L Q D P R V M T T L S V L L G V D L G S M D E E E E A A T P P P P P P P K K E P K P E P M E E D L P E N K K Q A L K E K E L G N D A Y K K K D F D K A L K H Y D R A K E L D P T N M T Y I T N Q A A V H F E K G D Y N K C R E L C E K A I E V G R E N R E D Y R Q I A K A Y A R I G N S Y F K E E K Y K D A H F Y N K S L A E H R T P D V L K K C Q Q A E K I L K E Q E R L A Y I N P D L A L E E K N K G N E C F Q K G D Y P Q A M K H Y T E A I K R N P R D A K L Y S N R A A C Y T K L L E F Q L A L K D C E E C I Q L E P T F I K G Y T R K A A A L E A M K D Y T K A M D V Y Q K A L D L D S S C K E A A D G Y Q R C M M A Q Y N R H D S P E D V K R R A M A D P E V Q Q I M S D P A M R L I L E Q M Q K D P Q A L S E H L K N P V I A Q K I Q K L M D V G L I A I R
- Accession
- Q60864
- Protein length
- Full Length
- Predicted molecular weight
- 64.7 kDa
- Amino acids
- 1 to 543
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
purified by using conventional chromatography techniques.
General info
- Function
Acts as a co-chaperone for HSP90AA1. Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90.
- Post-translational modifications
In vitro kinase assay failed to detect phosphorylation by MAPKAPK2.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as STIP1
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STIP1 also known as STI1 is a stress-induced phosphoprotein that assists in the folding and stabilization of other proteins. The protein has a mass of approximately 63 kDa. It acts as a co-chaperone and interacts with key chaperones like HSP70 and HSP90 facilitating the transfer of client proteins between them. STIP1 is expressed in various tissues with significant presence in the nervous system and reproductive organs. It contributes to the protein homeostasis by modulating chaperone activity and protecting cells during stress conditions like high temperatures or oxidative stress.
- Biological function summary
The stress-induced phosphoprotein STIP1 functions within cytoplasm and is pivotal for cellular integrity. It forms a complex with HSP70 and HSP90 that regulates the folding and activation of a range of client proteins including steroid hormone receptors and kinases. This modulation influences protein activity stability and degradation. STIP1 impacts signal transduction pathways cellular growth and stress responses by influencing how effectively client proteins perform their biological roles within cells.
- Pathways
The protein STIP1 plays a significant part in the steroid hormone signaling pathway and the protein folding network. It helps facilitate the proper folding and function of steroid hormone receptors through its interaction with major chaperone proteins HSP70 and HSP90. Both these pathways are important for cell survival and proper cell regulation. By helping with the maturation and activation of client proteins STIP1 ensures that cellular responses to various internal and external signals proceed efficiently.
- Associated diseases and disorders
The involvement of STIP1 emerges most clearly in neurodegenerative conditions like Alzheimer's disease and cancer. In Alzheimer's disease STIP1 interacts with prion proteins which influence amyloid-beta aggregation and may impact disease progression. In cancer the protein assists in stabilizing client proteins essential for tumor progression such as steroid hormone receptors and protein kinases. Disruption in STIP1 function can lead to impaired cellular stress responses and may contribute to disease pathogenesis.
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1 product image
SDS-PAGE - Recombinant Mouse STIP1/STI1 protein (His tag) (ab219449)
15% SDS-PAGE using 3μg of ab219449.
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