Recombinant Mouse sTNF RI Protein Standard (His tag)
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Recombinant Mouse sTNF RI Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
View Alternative Names
CD120a, Tnfr-1, Tnfr1, Tnfrsf1a, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I
- sELISA
Supplier Data
Sandwich ELISA - Recombinant Mouse sTNF RI Protein Standard (His tag) (AB316474)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Mouse sTNF RI Protein Standard (His tag) (AB316474)
SDS-PAGE analysis of ab316474 under reducing conditions for 2ug protein.
Reactivity data
Product details
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Soluble TNF Receptor I is involved in regulating inflammation. It sequesters TNF to control its bioavailability and impede its pro-inflammatory effects. sTNFR1 is part of a larger signaling complex which also includes other TNF receptors and ligands within the TNF receptor superfamily. This ability to regulate TNF activity suggests its important role in maintaining immune system balance and preventing excessive inflammation.
Pathways
Soluble TNF Receptor I modulates key inflammatory pathways including the NF-kB and MAPK pathways. These pathways are critical in the cellular response to stress apoptosis and survival. By binding TNF-alpha sTNFR1 interferes with TNF-alpha's interaction with TNFR1 and TNFR2 cell receptors which are vital in signaling through these pathways. TNFR1 particularly mediates a range of cellular responses including apoptosis and survival by influencing downstream kinases and transcription factors.
Specifications
Form
Liquid
General info
Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (By similarity).
Post-translational modifications
(Microbial infection) Glycosylated at Arg-376 by S.typhimurium protein Ssek3: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions.
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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